PROLONGED AND SIMPLIFIED INVITRO CULTURE OF EXPLANTED CHICK-EMBRYOS
PROLONGED AND SIMPLIFIED INVITRO CULTURE OF EXPLANTED CHICK-EMBRYOS
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DOI：
10.1007/bf00309751
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发表时间：
1987-01-01
期刊：
影响因子：
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通讯作者：
FLAMME, I
中科院分区：
文献类型：
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作者：
FLAMME, I
本文所描述的方法可以使鸡胚在体外培养的第2天至第5天正常发育。胚胎在一圈滤纸的帮助下外植，并将其固定在两个背面朝上的钢环之间进行培养。下环含有蛋黄和蛋白的混合物作为培养基。40%的外植胚胎发育到第5天（25期，Hamburger和Hamilton）。外植后最长培养时间为70 h。
The method described in this paper allows explanted chick embryos to develop normally in vitro from the 2nd to the 5th day of incubation. The embryos are explanted with the aid of a ring of filter-paper and are fixed between two steel-rings dorsal side up for cultivation. The lower ring contains a mixture of yolk and albumen as culture medium. 40% of the explanted embryos reach the 5th day of development (stage 25, Hamburger and Hamilton). The maximum time of culture is 70 h after explantation.