Critical appraisal of the mouse model of myocardial infarction
Critical appraisal of the mouse model of myocardial infarction
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DOI：
10.1113/expphysiol.2004.027276
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发表时间：
2004-07-01
影响因子：
2.7
通讯作者：
Alton, EWFW
中科院分区：
文献类型：
--
作者：
Degabriele, NM;Griesenbach, U;Alton, EWFW
为了严格评估心肌梗死 (MI) 小鼠模型在治疗研究中的实用性，我们研究了小鼠的存活率、血流动力学测量和组织病理学，并将闭塞缝合线放置在沿左冠状动脉前降支的三个不同部位之一。缝合线放置在房室交界处（高），或放置在更远离基底的两个部位（中和低）。在高组中，只有 33% 的动物在 MI 后 7 天存活（与所有其他组相比，P < 0.05）。与假手术动物相比，只有中间组的血流动力学显着降低（最大左心室压力：55.9 +/- 3.5 与 80.8 +/- 5.1 mmHg，随时间变化的最大压力变化：2003 +/- 172 与 4402 +/- 49 1，P < 0.01）。组织学检查显示所有 MI 组的形态学变化。中组病灶较低组更大（P < 0.05）。前壁和侧壁的病变与心脏功能相关，尽管相关性较弱。功效计算表明，尽管存在一定量的组内变异，中缝线模型可能对治疗研究有用，以评估治疗对心脏功能和总体病变大小的影响。
In order to critically evaluate the utility of a mouse model of myocardial infarction (MI) for therapeutic studies, we investigated survival, haemodynamic measurements and histopathology in mice with an occluding suture placed at one of three distinct sites along the left anterior descending coronary artery. The suture was placed at the atrioventricular juncture (High), or at two sites more distally towards the base (Middle and Low). In the High group, only 33% of animals survived 7 days after MI (P < 0.05 compared to all other groups). Only the Middle group had significantly reduced haemodynamics compared to sham-operated animals (maximum left ventricular pressure: 55.9 +/- 3.5 versus 80.8 +/- 5.1 mmHg, maximum change in pressure over time: 2003 +/- 172 versus 4402 +/- 49 1, P < 0.01). Histological examination showed morphological changes in all MI groups. The Middle group had larger lesions than the Low group (P < 0.05). Lesions in the anterior and lateral walls correlated, albeit weakly, with cardiac function. Power calculations indicated that, despite a certain amount of intragroup variation, the Middle Suture model may be useful for therapeutic studies to assess the effects of treatment on cardiac function and overall lesion size.