Studies of the response in mixed leukocyte culture of cells from patients with aplastic anemia to cells from HLA-identical siblings.
Studies of the response in mixed leukocyte culture of cells from patients with aplastic anemia to cells from HLA-identical siblings.
复制标题
研究再生障碍性贫血患者细胞的混合白细胞培养物对 HLA 相同同胞细胞的反应。
DOI：
10.1097/00007890-198108000-00002
复制
发表时间：
1981
期刊：
影响因子：
6.2
通讯作者：
Hansen,JA
中科院分区：
文献类型：
--
作者：
Mickelson,EM;Clift,RA;Fefer,A;Storb,R;Thomas,ED;Warren,RP;Hansen,JA
我们对64例重度再生障碍性贫血患者的混合白细胞培养（MLC）反应进行了研究。与来自正常hla相同的兄弟姐妹的细胞相比，他们的外周血单核细胞对hla相同的兄弟姐妹的细胞表现出更高的相对反应（RR），证实了早期34例患者系列报道的结果。通过抗体依赖细胞介导的细胞毒性试验检测，升高的RRs与患者抗供体淋巴细胞抗体相关，但与抗供体补体依赖的细胞毒性抗体或抗供体细胞毒性T淋巴细胞无关。在98例接受hla相同的兄弟姐妹骨髓移植的患者中，移植前rr升高的患者比未接受移植的患者更容易排斥移植（P< 0.0001）。10例未输血患者RR未升高，2例患者在首次输血后12 - 24小时内RR呈阳性。五名同卵双胞胎患者也接受了测试，其中三人的rr升高。虽然输血似乎是在一些再生患者中观察到的RRs增加的原因，但供体和受体之间的遗传差异并不总是引起MLC反应增加的必要条件，这表明除同种异体免疫外的其他机制也参与其中。
We have studied the mixed leukocyte culture (MLC) reactions of 64 patients with severe aplastic anemia. Their peripheral blood mononuclear cells showed an increased relative response (RR) to cells from HLA-identical siblings as compared to cells from normal HLA-identical siblings, confirming the results reported in an earlier series of 34 patients. Elevated RRs were associated with patient antidonor lymphocyte antibodies as detected by the antibody-dependent cell-mediated cytotoxicity assay, but were not associated with antidonor complement-dependent cytotoxic antibodies or with antidonor cytotoxic T lymphocytes. Among 98 patients receiving marrow grafts from HLA-identical sibling donors, those with elevated RRs before transplantation were more apt to reject the transplant than those without (P< 0.0001). There was no elevation of the RR in 10 untransfused patients, although positive RRs were noted in 2 patients within 12 to 24 hr of their first transfusions. Five patients with identical twins were also tested, and elevated RRs were noted in three. Although blood transfusion appears to be responsible for the increased RRs observed in some aplastic patients, genetic differences between donor and recipient were not always necessary for eliciting an increased MLC response, suggesting that mechanisms other than alloimmunization are involved.