Naive and memory T cells show distinct pathways of lymphocyte recirculation.
Naive and memory T cells show distinct pathways of lymphocyte recirculation.
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DOI：
10.1084/jem.171.3.801
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发表时间：
1990-03-01
期刊：
影响因子：
--
通讯作者：
Dudler L
中科院分区：
文献类型：
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作者：
Mackay CR;Marston WL;Dudler L
在本报告中，我们讨论了关于免疫记忆的两个问题：初始T细胞和记忆T细胞在体内循环的方式，以及初始T细胞和记忆T细胞群体内部分裂的内在速度。我们通过绵羊的细胞表面表型和对回忆抗原的反应能力鉴定了幼稚T细胞和记忆T细胞。记忆T细胞是CD2hi、CD58hi、CD44hi、CD11ahi和CD45R-，与人类有关。T细胞从血液中进入后肢组织，并在腘窝传入淋巴中积累，均为记忆表型。相反，传出淋巴中的T细胞，90%通过高内皮小静脉（HEV）进入淋巴结（LN），大多数为初始表型（CD2lo, CD58lo, CD44lo， CD11alo和CD45R+）。在不同的循环室中，这两种表型的显著富集表明，记忆T细胞选择性地从血液转运到外周组织（通过传入淋巴），而幼稚T细胞选择性地从血液转运到LN（通过HEV）。我们认为，这两种循环途径的不同使用可能优化了淋巴细胞与抗原的相互作用。T细胞亚群在各循环室中的非随机分布可能与各亚群中记忆细胞的相对比例有关。特别是，血液中的γ / δ T细胞几乎完全具有记忆表型，并且优先积聚在传入淋巴中，而不是传出淋巴中。最后，使用溴脱氧尿苷标记技术，我们发现至少相当大比例的记忆T细胞，无论是在血液中还是在传入淋巴中，都是一个分裂的细胞群，而幼稚T细胞是一个不分裂的细胞群。这一结果支持了另一种淋巴细胞记忆模型，该模型假设记忆的维持需要持续的抗原刺激。
In this report, we have addressed two questions concerning immunological memory: the way in which naive and memory T cells recirculate through the body, and the intrinsic rate of division within the naive and memory populations. We identified naive and memory T cells in sheep by their cell surface phenotype and their ability to respond to recall antigen. Memory T cells were CD2hi, CD58hi, CD44hi, CD11ahi, and CD45R-, as pertains in man. T cells that crossed from blood to the tissues of the hind leg and accumulated in the popliteal afferent lymph were all of memory phenotype. Conversely, T cells in efferent lymph, 90% of which entered the lymph node (LN) via high endothelial venules (HEV), were mostly of the naive phenotype (CD2lo, CD58lo, CD44lo, CD11alo, and CD45R+). The marked enrichment of these two phenotypes in different recirculatory compartments indicated that memory T cells selectively traffic from blood to peripheral tissues to LN (via afferent lymph), whereas naive T cells selectively traffic from blood to LN (via HEV). We argue that the differential use of these two recirculation pathways probably optimizes lymphocyte interactions with antigen. The nonrandom distribution of T cell subsets in various recirculatory compartments may be related to the relative proportion of memory cells in each subset. In particular, gamma/delta T cells in blood were almost exclusively of memory phenotype, and accumulated preferentially in afferent, but not in efferent, lymph. Finally, using the bromo-deoxyuridine labeling technique, we found that at least a sizeable proportion of memory T cells, whether in blood or afferent lymph, were a dividing population of cells, whereas naive T cells were a nondividing population. This result supports an alternative model of lymphocyte memory that assumes that maintenance of memory requires persistent antigenic stimulation.