Membrane-anchored CD14 is important for induction of interleukin-8 by lipopolysaccharide and peptidoglycan in uroepithelial cells
Membrane-anchored CD14 is important for induction of interleukin-8 by lipopolysaccharide and peptidoglycan in uroepithelial cells
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DOI：
10.1128/cdli.11.5.969-976.2004
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发表时间：
2004-09-01
期刊：
影响因子：
--
通讯作者：
Fujii, N
中科院分区：
文献类型：
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作者：
Shimizu, T;Yokota, S;Fujii, N
我们研究了细菌脂多糖（LPS）和肽聚糖（PGN）在膀胱癌细胞系T24、5637、UM-UC-3和HT 1197中诱导白细胞介素-8（IL-8）。T24和5637细胞在LPS或PGN刺激后以剂量和时间依赖的方式强烈诱导IL-8，而UM-UC-3和HT 1197细胞的诱导作用非常弱。CD 14在mRNA、总细胞蛋白和细胞表面蛋白水平上的表达在这些细胞系中不同，但Toll样受体2和4（TLR 2和TLR 4）的表达水平没有显著差异。CD 14的表达水平与LPS或PGN诱导的IL-8水平相关。用磷脂酰肌醇特异性磷脂酶C处理T24和5637细胞以从细胞表面消除CD 14显著抑制IL-8的诱导。另一方面，用CD 14 cDNA转染的UM-UC-3细胞表达膜锚定的CD 14，并显示出比未转染的对照组更有效的IL-8诱导。这些结果表明，存在膜锚定的，但不是可溶性的，形式的CD 14是一个强有力的因素，在膀胱上皮细胞的IL-8诱导响应细菌成分。因此，膜锚定形式的CD 14的存在可能是尿路上皮细胞炎症反应的决定因素。
We investigated the induction of interleukin-8 (IL-8) by bacterial lipopolysaccharide (LPS) and peptidoglycan (PGN) in the bladder cancer cell lines T24, 5637, UM-UC-3, and HT1197. T24 and 5637 cells strongly induced IL-8 after stimulation with LPS or PGN in a dose- and time-dependent manner, whereas UM-UC-3 and HT1197 cells did so very weakly. The expression of CD14 at the mRNA, total cellular protein, and cell surface protein levels differed among these cell lines, but the expression levels of Toll-like receptors 2 and 4 (TLR2 and TLR4) were not significantly different. The CD14 expression levels were found to correlate with the inducibility of IL-8 by LPS or PGN. Treatment of T24 and 5637 cells with phosphatidylinositol-specific phospholipase C to eliminate CD14 from the cell surface dramatically suppressed the induction of IL-8. On the other hand, UM-UC-3 cells transfected with CD14 cDNA expressed membrane-anchored CD14 and showed more efficent induction of IL-8 by LPS stimulation than untransfected controls. These results suggest that the presence of the membrane-anchored, but not the soluble, form of CD14 is a strong factor in IL-8 induction in bladder epithelial cells in response to bacterial components. The presence of the membrane-anchored form of CD14 may thus be a determinant for the inflammatory response of uroepithelial cells.