Strategies for developing DNA-encoded libraries beyond binding assays
Strategies for developing DNA-encoded libraries beyond binding assays
复制标题
开发结合测定之外的 DNA 编码文库的策略
DOI：
10.1038/s41557-021-00877-x
复制
发表时间：
2022-02-01
期刊：
影响因子：
21.8
通讯作者：
Li, Xiaoyu
中科院分区：
文献类型：
--
作者：
Huang, Yiran;Li, Yizhou;Li, Xiaoyu
DNA编码的化学文库(DELs)已成为药物发现中的一项强大技术。DEL在制药工业中的广泛采用和与DEL兼容的化学的快速进步进一步推动了它的发展和应用。一般来说，DEL被认为是一种大规模的结合分析，以确定单个蛋白质靶标的物理结合。然而，最近的创新证明了DELS在复杂的生物系统环境中运行的能力。在这一视角下，我们讨论了利用DNA编码的化学文库来询问复杂的生物靶标以及它们在识别具有功能或具有其他有用性质的结构方面的最新进展。未来在这些方面的突破有望使DEL成为一个重要的技术平台，不仅用于药物发现，而且还用于探索基础生物学。
DNA-encoded chemical libraries (DELs) have emerged as a powerful technology in drug discovery. The wide adoption of DELs in the pharmaceutical industry and the rapid advancements of DEL-compatible chemistry have further fuelled its development and applications. In general, a DEL has been considered as a massive binding assay to identify physical binders for individual protein targets. However, recent innovations demonstrate the capability of DELs to operate in the complex milieu of biological systems. In this Perspective, we discuss the recent progress in using DNA-encoded chemical libraries to interrogate complex biological targets and their potential to identify structures that elicit function or possess other useful properties. Future breakthroughs in these aspects are expected to catapult DEL to become a momentous technology platform not only for drug discovery but also to explore fundamental biology.