Determination of polyacetylenes and ginsenosides in Panax species using high performance liquid chromatography
Determination of polyacetylenes and ginsenosides in Panax species using high performance liquid chromatography
复制标题
DOI：
10.1248/cpb.51.1314
复制
发表时间：
2003-11-01
影响因子：
1.7
通讯作者：
Kitanaka, S
中科院分区：
文献类型：
--
作者：
Washida, D;Kitanaka, S
建立了一种新的分离和鉴定人参属植物中的三种聚乙炔(人参醇、人参醇和1，8-十七二烯-4，6-二炔-3，10-二醇)的高效液相色谱方法。流动相为2：1：3~2：1：1(v/v/v)甲醇/乙腈/水的线性梯度为40min。高效液相色谱法，流速1.5ml/min，检测波长254 nm。用这些方法测定了人参(白参和红参)、西洋参、刺参和三七中多乙炔和人参皂苷的含量。聚乙炔含量最高(0.080%)的树种是日本长野栽培的西洋参。同时，人参皂苷含量最高(9.176%)的品种是云南栽培的三七中国。
A new HPLC method was developed to separate and identify three polyacetylenes (panaxynol, panaxydol and 1,8-heptadecadiene-4,6-diyne-3,10-diol) found in Panax species. The mobile phase was a linear gradient of 2:1:3 to 2:1:1 (v/v/v) methanol/acetonitrile/water in 40 min. HPLC analysis was performed at a flow rate of 1.5 ml/min with UV detection at 254 nm. The contents of the polyacetylenes and ginsenosides in Panax ginseng (white ginseng and red ginseng), R quinquefolium, R japonicus, and P. noteginseng were determined using these methods. The species containing the highest polyacetylene content (0.080%) was R quinquefolium cultivated in Nagano, Japan. Meanwhile, the species with the highest ginsenoside content (9.176%) was P noteginseng cultivated in Yunnan, China.