Analysis of high-molecular-weight fructan polymers in crude plant extracts by high-resolution LC-MS.
Analysis of high-molecular-weight fructan polymers in crude plant extracts by high-resolution LC-MS.
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通过高分辨率LC-MS在粗植物提取物中分析高分子重量果糖聚合物。
DOI：
10.1007/s00216-011-5374-8
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发表时间：
2011-11
影响因子：
4.3
通讯作者：
Rasmussen, Susanne
中科院分区：
文献类型：
--
作者：
Harrison, Scott;Fraser, Karl;Lane, Geoff;Hughes, Daniel;Villas-Boas, Silas;Rasmussen, Susanne
温带牧草中的主要水溶性碳水化合物是多聚果糖。果聚糖由不同链长的果糖链组成，作为核心分子附着在蔗糖上。在牧草中，果聚糖是大量异构型低聚物的复杂混合物，聚合度从3到>；100不等。准确的监测和明确的峰识别需要将色谱分离与质谱分析相结合。离子陷阱质谱仪的质量范围是有限的，我们在这里展示了如何利用监测选定的多电荷离子来检测和定量复杂植物提取物中的单个高质量异构体和低聚物，特别是那些高聚合度的异构体和低聚物(DP >； 20)。以前报道的使用低质量分辨率的线性离子陷阱的方法已被证明对于检测聚合度高达49的果聚糖是有用的。在这里，我们报告了一种使用高分辨率质谱仪(MS)的方法，该方法使用Exactive Orbitrap MS，大大提高了信噪比，并允许检测高达DP = 100的果聚糖。含有高浓度果聚糖的多年生黑麦草品种已被证明有利于畜牧业，因为它们提高了瘤胃氮的利用效率，减少了牧场的一氧化二氮排放。我们用我们的方法证明了这些HS草不仅在叶片中含有更多的果聚糖，而且与正常糖水平的品种相比，还积累了更高DP的果聚糖。本文的在线版本(doi：10.1007/s002160115374-8)包含补充材料，授权用户可以使用。
The main water-soluble carbohydrates in temperate forage grasses are polymeric fructans. Fructans consist of fructose chains of various chain lengths attached to sucrose as a core molecule. In grasses, fructans are a complex mixture of a large number of isomeric oligomers with a degree of polymerisation ranging from 3 to >100. Accurate monitoring and unambiguous peak identification requires chromatographic separation coupled to mass spectrometry. The mass range of ion trap mass spectrometers is limited, and we show here how monitoring selected multiply charged ions can be used for the detection and quantification of individual isomers and oligomers of high mass, particularly those of high degree of polymerization (DP > 20) in complex plant extracts. Previously reported methods using linear ion traps with low mass resolution have been shown to be useful for the detection of fructans with a DP up to 49. Here, we report a method using high-resolution mass spectrometry (MS) using an Exactive Orbitrap MS which greatly improves the signal-to-noise ratio and allows the detection of fructans up to DP = 100. High-sugar (HS) Lolium perenne cultivars with high concentrations of these fructans have been shown to be of benefit to the pastoral agricultural industry because they improve rumen nitrogen use efficiency and reduce nitrous oxide emissions from pastures. We demonstrate with our method that these HS grasses not only contain increased amounts of fructans in leaf blades but also accumulate fructans with much higher DP compared to cultivars with normal sugar levels. The online version of this article (doi:10.1007/s00216-011-5374-8) contains supplementary material, which is available to authorized users.
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影响因子：
3.5
作者：
Ellis, J. L.;Dijkstra, J.;France, J.
通讯作者：
France, J.
影响因子：
1
作者：
TIMMERMANS, JW;VANLEEUWEN, MB;VLIEGENTHART, JFG
通讯作者：
VLIEGENTHART, JFG
影响因子：
6.9
作者：
Tamura K;Kawakami A;Sanada Y;Tase K;Komatsu T;Yoshida M
通讯作者：
Yoshida M
影响因子：
4.1
作者：
BACON, JSD
通讯作者：
BACON, JSD
影响因子：
9.4
作者：
LEWIS, DH
通讯作者：
LEWIS, DH