Comparative expression of RANK, RANKL, and OPG in keratocystic odontogenic tumors, ameloblastomas, and dentigerous cysts
Comparative expression of RANK, RANKL, and OPG in keratocystic odontogenic tumors, ameloblastomas, and dentigerous cysts
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DOI：
10.1016/j.tripleo.2007.06.009
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发表时间：
2008-03-01
期刊：
影响因子：
--
通讯作者：
Cunha, Fernando Queiroz
中科院分区：
文献类型：
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作者：
da Silva, Tarcilia Aparecida;Batista, Aline Carvalho;Cunha, Fernando Queiroz
Objective.本研究的目的是比较核因子κ B（RANK）、RANK配体（RANKL）和骨保护素（OPG）在牙源性上皮肿瘤和含牙囊肿中的表达。采用免疫组化法检测这些分子在12例实性/多囊性成釉细胞瘤、8例单囊性成釉细胞瘤、19例角化囊性牙源性肿瘤、9例含牙囊肿和9例牙囊中的表达。在牙源性上皮中，在所有样本中观察到RANK、RANKL和OPG的相似表达。在基质方面，实性/多囊性成釉细胞瘤中RANK阳性和RANKL阳性细胞的数量高于含牙囊肿。牙囊中RANK阳性、RANKL阳性和OPG阳性细胞的数量低于实性/多囊性成釉细胞瘤和角化囊性牙源性肿瘤。大多数实性/多囊性成釉细胞瘤（75%）和单囊性成釉细胞瘤（62.5%）的RANKL阳性细胞数高于OPG阳性细胞数。而62.4%的牙源性角化囊性肿瘤和100%的含牙囊肿中OPG阳性细胞含量高于RANKL阳性细胞。我们的研究结果表明，RANK，RANKL和OPG的表达在牙源性上皮肿瘤的差异。这些因素的不平衡可能导致这些病变中骨/牙吸收活性的差异。
Objective. The aim of this study was to compare the expression of nuclear factor kappa B (RANK), RANK ligand (RANKL), and osteoprotegerin (OPG) in odontogenic epithelial tumors and dentigerous cysts.Study design. The expression of these molecules was evaluated in solid/ multicystic ameloblastomas (n = 12) and unicystic ameloblastomas (n = 8), keratocystic odontogenic tumors (n = 19), dentigerous cysts (n = 9), and dental follicles (n = 9) by immunohistochemistry.Results. In odontogenic epithelium, a similar expression of RANK, RANKL, and OPG was observed in all samples. With regard to stroma, the number of RANK-positive and RANKL-positive cells was higher in solid/ multicystic ameloblastomas compared with dentigerous cysts. Dental follicles had lower numbers of RANK-positive, RANKL-positive, and OPG-positive cells than solid/ multicystic ameloblastomas and keratocystic odontogenic tumors. The majority of solid/ multicystic ameloblastomas (75%) and unicystic ameloblastomas (62.5%) had higher numbers of RANKL-positive than OPG-positive cells. In contrast, 62.4% of keratocystic odontogenic tumors and 100% of dentigerous cysts exhibited a higher content of OPG-positive than RANKL-positive cells.Conclusion. Our results indicate differences in the RANK, RANKL, and OPG expression in odontogenic epithelial tumors. The imbalance of these factors could contribute to the differential bone/ tooth resorption activity in these lesions.