Human Prolactin Promotes Human Secondary Immunoglobulin Response in Human/SCID Mouse Chimeras
Human Prolactin Promotes Human Secondary Immunoglobulin Response in Human/SCID Mouse Chimeras
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DOI：
10.1128/cvi.00244-06
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发表时间：
2006-11
影响因子：
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通讯作者：
Jian Zhang;R. Sun;Z. Tian
中科院分区：
文献类型：
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作者：
Jian Zhang;R. Sun;Z. Tian
用重组人催乳素（rhPRL）对huPBL-SCID小鼠进行免疫刺激，观察其对人免疫球蛋白（IG）产生的影响。将huPBL-SCID小鼠腹膜内（i. p.）每隔一天注射10 μg rhPRL，共注射10次。结果再次证实，rhPRL显著增加了huPBL-SCID小鼠脾脏、淋巴结和胸腺中人CD 3 + T细胞和人CD 19 + B细胞的数量。然后同时给予huPBL-SCID小鼠不同剂量的白喉-破伤风（DT）疫苗和10 μg rhPRL腹腔注射，并检查体外淋巴结细胞的人DT特异性增殖和体内抗体产生。rhPRL极大地改善了DT免疫的huPBL-SCID小鼠中功能性人淋巴细胞（CD 3 + T细胞和CD 19 + B细胞）的植入。rhPRL处理的DT免疫的huPBL-SCID小鼠响应于疫苗产生显著更大量的DT特异性抗体。免疫后诱导的主要IG同种型为IgG。因此，rhPRL刺激促进huPBL-SCID小鼠中的人二次IgG应答。
ABSTRACT Recombinant human prolactin (rhPRL) was administered to huPBL-SCID mice to determine its effects on production of human immunoglobulin (Ig). The huPBL-SCID mice were injected intraperitoneally (i.p.) with 10 μg rhPRL every other day for a total of 10 injections. The results reconfirmed that rhPRL significantly increased the numbers of human CD3+ T cells and human CD19+ B cells in spleens, lymph nodes, and thymuses of huPBL-SCID mice. The huPBL-SCID mice were then concurrently given various doses of diphtheria-tetanus (DT) vaccine and 10-μg i.p. injections of rhPRL and were examined for the presence of human DT-specific proliferation of lymph node cells in vitro and antibody production in vivo. rhPRL greatly improved the engraftment of functional human lymphocytes (CD3+ T cells and CD19+ B cells) in DT-immunized huPBL-SCID mice. The rhPRL-treated, DT-immunized huPBL-SCID mice produced significantly larger amounts of DT-specific antibodies in response to the vaccine. The predominant Ig isotype induced after immunization was IgG. Thus, rhPRL stimulation promotes human secondary IgG responses in huPBL-SCID mice.