Comparison of the lateral tail vein and the retro-orbital venous sinus as routes of intravenous drug delivery in a transgenic mouse model
Comparison of the lateral tail vein and the retro-orbital venous sinus as routes of intravenous drug delivery in a transgenic mouse model
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DOI：
10.1038/laban0108-26
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发表时间：
2008-01-01
期刊：
影响因子：
6.9
通讯作者：
Ciavarra, Richard R.
中科院分区：
文献类型：
--
作者：
Steel, Christina D.;Stephens, Amber L.;Ciavarra, Richard R.
在小鼠中，通常通过尾侧静脉进行静脉注射。这项技术有时很难实施，可能会给老鼠带来压力。虽然通过眶后静脉窦注射比侧尾静脉注射有一定的优势，但这种方法定义不明确，很少使用。为了比较两种给药途径的疗效，作者给MAFIA转基因小鼠注射了选择性诱导巨噬细胞凋亡的消耗剂AP20187。每只小鼠通过尾侧静脉或眶后静脉窦连续每天注射5次。然后，作者比较了巨噬细胞在不同组织（肺、脾、骨髓和腹膜渗出细胞）中的损耗。两种注射方式的效果相似。另一项BALB/c小鼠实验表明，眶后静脉窦注射是两种方法中压力较小的一种。
In mice, intravenous injections are commonly administered in the lateral tail vein. This technique is sometimes difficult to carry out and may cause stress to mice. Though injection through the retro-orbital venous sinus can provide certain advantages over lateral tail vein injection, this method is poorly defined and infrequently used. To compare the efficacy of these two routes of drug delivery, the authors injected MAFIA transgenic mice with the depletion agent AP20187, which selectively induces apoptosis in macrophages. Each mouse received five consecutive daily injections through either the Lateral tail vein or the retro-orbital venous sinus. The authors then compared macrophage depletion in different tissues (lung, spleen, bone marrow and peritoneal exudate cells). Both routes of injection were similarly effective. A separate experiment using BALB/c mice indicated that retro-orbital venous sinus injection was the less stressful of the two methods.