Characterization of lipoprotein particles isolated by immunoaffinity chromatography. Particles containing A-I and A-II and particles containing A-I but no A-II.
Characterization of lipoprotein particles isolated by immunoaffinity chromatography. Particles containing A-I and A-II and particles containing A-I but no A-II.
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发表时间：
1984-10
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通讯作者：
M. Cheung;J. Albers
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作者：
M. Cheung;J. Albers
用免疫亲和层析法从10名血脂正常受试者的血浆中分离出两种含A-I的脂蛋白颗粒：含A-I的脂蛋白和A-II（Lp（A-I和A-II）），和含A-I的脂蛋白和不含A-II（Lp（A-I和A-II））。这两种类型的颗粒具有α-电泳迁移率和水合密度的范围内的血浆高密度脂蛋白（HDL）。Lp（A-I无A-II）和Lp（A-I有A-II）大小不均匀。Lp（A-I无A-II）包含两种不同的粒度，其中Lp（A-I无A-II）1的平均表观分子量和斯托克斯直径为3.01 × 10（5）和10.8 nm，Lp（A-I无A-II）2的平均表观分子量和斯托克斯直径为1.64 × 10（5）和8.5 nm。Lp（A-I和A-II）通常含有至少三种不同分子尺寸的颗粒，Lp（A-I和A-II）1的平均表观分子量和斯托克斯直径分别为2.28 × 10（5）和9.6 nm，Lp（A-I和A-II）2的平均表观分子量和斯托克斯直径分别为1.80 × 10（5）和8.9 nm，Lp（A-I和A-II）3的平均表观分子量和斯托克斯直径分别为1.25 × 10（5）和8.0 nm。在Lp（A-I无A-II）和Lp（A-I伴A-II）中均检测到载脂蛋白C、D和E以及卵磷脂：胆固醇酰基转移酶（LCAT），大部分载脂蛋白D和E以及LCAT（EC 2.3.1.43）在Lp（A-I伴A-II）颗粒中。Lp（A-I，无A-II）的脂质/蛋白质比略高于Lp（A-I，有A-II）。Lp（A-I与A-II）的A-I/A-II摩尔比约为2：1。Lp相关的血浆A-I（A-I不含A-II）百分比与血浆A-I/A-II比值高度相关（r = 0.96，n = 10）。因此，HDL密度亚组分的A-I/A-II比率的变化反映了两种离散类型颗粒的不同比例：以几乎恒定的比率含有A-I和A-II的颗粒和含有A-II但不含A-II的颗粒。每种类型的颗粒在大小和脱辅基蛋白组成上是异质的。
Two populations of A-I-containing lipoprotein particles: A-I-containing lipoprotein with A-II (Lp (A-I with A-II], and A-I-containing lipoprotein without A-II (Lp (A-I without A-II] have been isolated from plasma of 10 normolipidemic subjects by immunoaffinity chromatography and characterized. Both types of particles possess alpha-electrophoretic mobility and hydrated density in the range of plasma high-density lipoproteins (HDL). Lp (A-I without A-II) and Lp (A-I with A-II) are heterogeneous in size. Lp (A-I without A-II) comprised two distinct particle sizes with mean apparent molecular weight and Stokes diameter of 3.01 X 10(5), and 10.8 nm for Lp (A-I without A-II)1, and 1.64 X 10(5), and 8.5 nm for Lp (A-I without A-II)2. Lp (A-I with A-II) usually contained particles of at least three distinct molecular sizes with mean apparent molecular weight and Stokes diameter of 2.28 X 10(5) and 9.6 nm for Lp (A-I with A-II)1, 1.80 X 10(5) and 8.9 nm for Lp (A-I with A-II)2, and 1.25 X 10(5) and 8.0 nm for Lp (A-I with A-II)3. Apoproteins C, D, and E, and lecithin:cholesterol acyltransferase (LCAT) were detected in both Lp (A-I without A-II) and Lp (A-I with A-II) with most of the apoprotein D, and E, and LCAT (EC 2.3.1.43) in Lp (A-I with A-II) particles. Lp (A-I without A-II) had a slightly higher lipid/protein ratio than Lp (A-I with A-II). Lp (A-I with A-II) had an A-I/A-II molar ratio of approximately 2:1. The percentage of plasma A-I associated with Lp (A-I without A-II) was highly correlated with the A-I/A-II ratio of plasma (r = 0.96, n = 10). The variation in A-I/A-II ratio of HDL density subfractions therefore reflects different proportions of two discrete types of particles: particles containing A-I and A-II in a nearly constant ratio and particles containing A-II but no A-II. Each type of particle is heterogeneous in size and in apoprotein composition.