Functional, anatomical, and neurochemical differentiation of medial preoptic area subregions in relation to maternal behavior in the mouse
Functional, anatomical, and neurochemical differentiation of medial preoptic area subregions in relation to maternal behavior in the mouse
复制标题
DOI：
10.1002/cne.23251
复制
发表时间：
2013-05
影响因子：
2.5
通讯作者：
Yousuke Tsuneoka;Teppo Maruyama;S. Yoshida;K. Nishimori;Tadafumi Kato;M. Numan;Kumi O. Kuroda
中科院分区：
文献类型：
--
作者：
Yousuke Tsuneoka;Teppo Maruyama;S. Yoshida;K. Nishimori;Tadafumi Kato;M. Numan;Kumi O. Kuroda
在啮齿类动物中，先前的研究结果表明内侧视前区（MPOA）在母性行为的神经控制中的重要参与。然而，特定的MPOA亚区参与母性行为和识别的神经化学表型（S）的必需神经元的规格需要额外的研究。因此，我们研究了化学神经解剖学的基本MPOA亚区的母性行为在C57 BL/6 J雌性小鼠。使用背侧MPOA中的催产素能神经元作为主要区域标记，我们首先使用免疫组织化学评估了母性行为期间MPOA中表达c-Fos的神经元的分布。结果表明，在母性行为过程中，背侧和腹侧MPOA的非催产素能神经元显著表达c-Fos。然后使用兴奋性毒性损伤研究，我们确定了特定的MPOA区域，这是必要的母性行为。中央MPOA的双侧病变（其中c-Fos仅中度表达）有效地扰乱了母体行为，尽管背侧和腹侧MPOA区域的病变无效。这些中央病变的女性是高度杀精，无论他们以前的母亲的经验。神经化学研究表明，中枢MPOA中超过75%的c-Fos-表达神经元是GABA能的。他们中的许多人也表达甘丙肽，神经降压素，和/或速激肽2的mRNA。最后，中央MPOA由许多神经元组成，尽管这些神经元中只有一小部分与c-Fos共定位。总之，中央MPOA是小鼠母性行为不可或缺的亚区，该区域的GABA能和/或肽能神经元在母性行为过程中被转录激活。J. Comp.神经元521：1633-1663，2013.© 2012 Wiley Periodicals，Inc.
In rodents, previous findings indicate critical involvement of the medial preoptic area (MPOA) in the neural control of maternal behavior. However, the specification of the particular MPOA subregions involved in maternal behavior and the identification of the neurochemical phenotype(s) of the essential neurons demands additional study. Therefore, we investigated the chemical neuroanatomy of the essential MPOA subregion for maternal behavior in C57BL/6J female mice. Using the oxytocinergic neurons in the dorsal MPOA as a primary regional marker, we first assessed the distribution of c‐Fos‐expressing neurons in the MPOA during maternal behavior using immunohistochemistry. Results showed that non‐oxytocinergic neurons in the dorsal and ventral MPOA prominently expressed c‐Fos during maternal behavior. Then using excitotoxic lesion studies, we determined the specific MPOA area that is necessary for maternal behavior. Bilateral lesions of the central MPOA, where c‐Fos was expressed only moderately, effectively disrupted maternal behavior, although lesions to the dorsal and ventral MPOA regions were ineffective. These centrally lesioned females were highly infanticidal irrespective of their previous maternal experience. Neurochemical investigations showed that more than 75% of the c‐Fos‐expressing neurons in central MPOA were GABAergic. Many of them also expressed galanin, neurotensin, and/or tachykinin2 mRNAs. Finally, the central MPOA was populated by numerous glutamatergic neurons, although only a small percentage of these neurons colocalized with c‐Fos. To conclude, the central MPOA is the indispensable subregion for mouse maternal behavior, and GABAergic and/or peptidergic neurons in this area were transcriptionally activated during maternal behavior. J. Comp. Neurol. 521:1633–1663, 2013. © 2012 Wiley Periodicals, Inc.