A beginner's guide to radiation damage.
A beginner's guide to radiation damage.
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DOI：
10.1107/s0909049509004361
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发表时间：
2009-03
影响因子：
2.5
通讯作者：
Holton JM
中科院分区：
文献类型：
--
作者：
Holton JM
辐射损伤的考虑影响数据收集的两个以上的因素进行了总结，并提出了避免损害的策略。近年来，在理解蛋白质晶体的辐射损伤方面取得了许多进展，特别是在低温下，但随之而来的是不断扩大的文献，对于新一代的蛋白质晶体学家来说，他们对X射线物理学或辐射化学并不真正感兴趣，只是想解决生物学相关的结构，这些文献的技术性质和广度可能令人望而生畏。本文旨在对辐射损伤问题提供一个粗略的指导，并为更精确和详细的工作提供参考。没有试图报告精确的数字（两个因素被认为是令人满意的），而且，由于有方面的辐射损害是明显不可预测的，“最坏的情况下”，以及“平均晶体”的数据收集的实用性方面进行了讨论。
Radiation damage considerations affecting data collection by more than a factor of two are summarized and damage avoidance strategies are suggested. Many advances in the understanding of radiation damage to protein crystals, particularly at cryogenic temperatures, have been made in recent years, but with this comes an expanding literature, and, to the new breed of protein crystallographer who is not really interested in X-ray physics or radiation chemistry but just wants to solve a biologically relevant structure, the technical nature and breadth of this literature can be daunting. The purpose of this paper is to serve as a rough guide to radiation damage issues, and to provide references to the more exacting and detailed work. No attempt has been made to report precise numbers (a factor of two is considered satisfactory), and, since there are aspects of radiation damage that are demonstrably unpredictable, the ‘worst case scenario’ as well as the ‘average crystal’ are discussed in terms of the practicalities of data collection.