Sensitive quantification of BB-22 and its metabolite BB-22 3-carboxyindole, and characterization of new metabolites in authentic urine and/or serum specimens obtained from three individuals by LC-QTRAP-MS/MS and high-resolution LC-Orbitrap-MS/MS
Sensitive quantification of BB-22 and its metabolite BB-22 3-carboxyindole, and characterization of new metabolites in authentic urine and/or serum specimens obtained from three individuals by LC-QTRAP-MS/MS and high-resolution LC-Orbitrap-MS/MS
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DOI：
10.1007/s11419-018-0448-3
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发表时间：
2019-01-01
影响因子：
2.2
通讯作者：
Watanabe, Kanako
中科院分区：
文献类型：
--
作者：
Minakata, Kayoko;Hasegawa, Koutaro;Watanabe, Kanako
目的人工合成大麻素BB-22及其代谢物BB-22 3-羧基吲哚在人体尿液中尚未被检测到。本研究的目的是建立一种灵敏的测定人血清和尿样中BB-22及其3-羧基吲哚的分析方法，并对3个个体的真实尿样中未见报道的BB-22代谢物进行表征。结果尿中BB-22和BB-22 3-羧基吲哚的检出限分别为3和30pg/mL。用该方法对两名吸毒者的正品血清和尿液以及一名吸毒者的尿液中的这些化合物进行了定量。病例1和病例2的血清BB-22水平分别为149pg/m L和6680pg/m L，BB-22 3-羧基吲哚分别为0.755和38.0 ng/m L。病例1、例2和例3的尿中BB-22含量分别为5.64、5.52和6.92pg/mL，3-羧基吲哚含量分别为0.131、21.4和5.15 ng/mL。结论首次在真实的人血清和尿样中鉴定和定量了合成的大麻素BB-22及其代谢物BB-22的3-羧基吲哚，并在3名吸毒者的尿样中初步鉴定了BB-22的新代谢物。
PurposeA synthetic cannabinoid BB-22 and its metabolite BB-22 3-carboxyindole have not yet been quantifiedin human urine. The aim of this study is to establish a sensitive analytical method for the quantification of BB-22 and its 3-carboxyindole in human serum and urine specimens, and the characterization of the unreported metabolites of BB-22 in authentic urine specimens from three individuals.MethodsThese compounds were extracted from -glucuronide-hydrolyzed and unhydrolyzed urine and/or serum via liquid-liquid extraction. The identification and quantification were performed using liquid chromatography (LC)-QTRAP-tandem mass spectrometry (MS/MS) and the characterization of the new metabolites was made by high-resolution LC-MS/MS.ResultsThe limits of detection of BB-22 and BB-22 3-carboxyindole were 3 and 30pg/mL in urine, respectively. The devised method was applied to quantify these compounds in authentic serum and urine obtained from two drug abusers and in urine from one drug abuser. The serum levels of BB-22 were 149 and 6680pg/mL, and those of BB-22 3-carboxyindole were 0.755 and 38.0ng/mL in cases 1 and 2, respectively. The urine levels of BB-22 were 5.64, 5.52 and 6.92pg/mL and those of BB-22 3-carboxyindole were 0.131, 21.4 and 5.15ng/mL in cases 1, 2 and 3, respectively. New monohydroxyl metabolites retaining the structure of BB-22 were found in the urine specimens.ConclusionsThe synthetic cannabinoid BB-22 and its metabolite BB-22 3-carboxyindole were identified and quantified in authentic human serum and urine specimens for the first time, and new metabolites of BB-22 were tentatively identified in authentic urine specimens obtained from three drug usersin this study.