Establishment of four pairs of canine mammary tumour cell lines derived from primary and metastatic origin and their E-cadherin expression
Establishment of four pairs of canine mammary tumour cell lines derived from primary and metastatic origin and their E-cadherin expression
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DOI：
10.1111/j.1476-5810.2006.00098.x
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发表时间：
2006-06-01
影响因子：
2.1
通讯作者：
Sasaki, N.
中科院分区：
文献类型：
--
作者：
Uyama, R.;Nakagawa, T.;Sasaki, N.
建立了4对来源于原发灶和转移灶的犬乳腺癌细胞系。在含10%胎牛血清的RPMI-1640培养液中，细胞生长稳定，传代超过120代。用流式细胞仪检测E-钙粘附素的表达，用细胞聚集法检测E-钙粘附素的功能，并对原发灶和转移灶的结果进行统计学比较。E-钙粘附素在所有细胞系中都有强烈表达，原发和转移来源的细胞之间没有显著差异。在细胞聚集实验中，原发源细胞中E-钙粘附素的功能明显弱于转移源细胞(p<；0.05)。目前的结果提示E-钙粘蛋白功能的降低可能与犬乳腺癌细胞的侵袭和转移有关；然而，还需要进一步的研究来阐明E-钙粘蛋白在犬乳腺癌转移中的作用。
Four new pairs of canine mammary carcinoma cell lines derived from both primary and metastatic lesions were established. The cells were cultured in RPMI-1640 with 10% fetal bovine serum and they showed stable growth for more than 120 passages. Using these cell lines, the expression of E-cadherin was measured by flow cytometry and the function of E-cadherin was evaluated by cell aggregation assay and results from the primary and metastatic lesions were compared statistically. E-cadherin was strongly expressed in all of the cell lines, without a notable difference between cells of primary and metastatic origin. In the cell aggregation assay, the function of E-cadherin was significantly weaker in the cells of primary origin (p < 0.05), as compared with cells of metastatic origin. The present results suggest that a reduction in E-cadherin function may be implicated in the invasive and metastatic potential of canine mammary tumour cells; however, further study will be needed to clarify E-cadherin function in the context of the metastasis of canine mammary carcinoma.