Green tea polyphenols induce differentiation and proliferation in epidermal keratinocytes
Green tea polyphenols induce differentiation and proliferation in epidermal keratinocytes
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DOI：
10.1124/jpet.103.049734
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发表时间：
2003-07-01
影响因子：
3.5
通讯作者：
Schuster, G
中科院分区：
文献类型：
--
作者：
Hsu, S;Bollag, WB;Schuster, G
最丰富的绿茶多酚，表没食子儿茶素没食子酸酯(EGCG)，被发现在肿瘤细胞和正常细胞之间诱导不同的作用。然而，正常的上皮细胞如何在肿瘤细胞发生凋亡的浓度下对多酚做出反应尚不清楚。目前的研究测试了原代人类表皮角质形成细胞对EGCG或四种主要绿茶多酚混合物的指数生长和老化反应。通过角蛋白1、微丝蛋白的表达和转谷氨酰胺酶活性的测定，EGCG在24小时内诱导生长角质形成细胞分化并联合诱导p57/KIP2。衰老的角质形成细胞在生长介质中培养25天后表现出较低的基础细胞活性，在EGCG或多酚的作用下，其DNA合成和琥珀酸脱氢酶活性最高可达37倍。这些结果表明，茶多酚可用于治疗伤口或某些以细胞活动或新陈代谢改变为特征的皮肤疾病。
The most abundant green tea polyphenol, epigallocatechin-3-gallate (EGCG), was found to induce differential effects between tumor cells and normal cells. Nevertheless, how normal epithelial cells respond to the polyphenol at concentrations for which tumor cells undergo apoptosis is undefined. The current study tested exponentially growing and aged primary human epidermal keratinocytes in response to EGCG or a mixture of the four major green tea polyphenols. EGCG elicited cell differentiation with associated induction of p57/KIP2 within 24 h in growing keratinocytes, measured by the expression of keratin 1, filaggrin, and transglutaminase activity. Aged keratinocytes, which exhibited low basal cellular activities after culturing in growth medium for up to 25 days, renewed DNA synthesis and activated succinate dehydrogenase up to 37-fold upon exposure to either EGCG or the polyphenols. These results suggest that tea polyphenols may be used for treatment of wounds or certain skin conditions characterized by altered cellular activities or metabolism.