Solution structure of TA1092, a ribosomal protein S24e from Thermoplasma acidophilum
Solution structure of TA1092, a ribosomal protein S24e from Thermoplasma acidophilum
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DOI：
10.1002/prot.21045
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发表时间：
2006-09-01
影响因子：
2.9
通讯作者：
Lee, Weontae
中科院分区：
文献类型：
--
作者：
Jeon, Byoung-Young;Jung, Jinwon;Lee, Weontae
导论.核糖体是催化蛋白质合成的大型核糖核蛋白复合物。在古细菌中，核糖体由一个小的30 S亚基和一个大的50 S亚基组成。古细菌核糖体的30 S亚基由一个16 SrRNA和28个核糖体蛋白组成。编码核糖体蛋白的基因由多个加工假基因组成，它们分散在基因组中。核糖体结构的最新进展1表明核糖体亚基内rRNA和核糖体蛋白之间存在密切的分子相互作用。然而，在翻译的各个阶段，核糖体成分的结构作用仍有待确定。本文报道了嗜酸热浆菌S24 e蛋白家族成员TA 1092的溶液结构。2 S24 e蛋白家族成员是古生菌和真核生物中30 S亚基的组成部分。由于核糖体蛋白S24 e的详细结构尚未用于模式生物，TA 1092的结构将扩展我们对不同物种的30 S核糖体蛋白结构-功能的知识和理解。将TA 1092基因克隆到表达载体pET 15 b中，并在大肠杆菌菌株BL 21（DE 3）（Novagen Inc.，麦迪逊，威斯康星州）。细胞在含有15 NH 4Cl和13 C-葡萄糖的基本M9培养基上生长，以获得均匀标记的细胞。
Introduction. The ribosome is a large ribonucleoprotein complex that catalyzes protein synthesis. In archaea, the ribosome is composed of a small 30S subunit and a large 50S subunit. The 30S subunit of the archaea ribosome is composed of a 16S rRNA and 28 ribosomal proteins. Genes that encode ribosomal proteins consist of multiple processed pseudogenes, which are dispersed through the genome. Recent advances in ribosome structure1 suggest close molecular interactions between rRNA and ribosomal protein, within ribosomal subunits. However, the structural role of ribosomal components during various stages of translation remains to be characterized. Here we report the solution structure of TA1092, a member of the S24e protein family from Thermoplasma acidophilum. 2 Members of the S24e protein family are components of the 30S subunit in both archaea and eukaryotes. Because the detailed structure of ribosomal protein S24e is not yet available for model organisms, the structure of TA1092 will expand our knowledge and understanding of 30S ribosomal protein structure–function for different species.Methods and Materials. The TA1092 gene was cloned into the expression vector, pET15b, and overexpressed in Escherichia coli strain BL21 (DE3)(Novagen Inc., Madison, WI). The cells were grown on a minimal M9 medium, with 15NH4Cl and 13C-glucose to obtain uniformly labeled