Genes involved in fatty acid metabolism: Molecular characterization and hypothalamic mRNA response to energy status and neuropeptide Y treatment in the orange-spotted grouper Epinephelus coioides
Genes involved in fatty acid metabolism: Molecular characterization and hypothalamic mRNA response to energy status and neuropeptide Y treatment in the orange-spotted grouper Epinephelus coioides
复制标题
参与脂肪酸代谢的基因：橙色斑点石斑鱼斜带石斑鱼能量状态和神经肽 Y 治疗的分子特征和下丘脑 mRNA 反应
DOI：
10.1016/j.mce.2013.06.020
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发表时间：
2013-08-25
影响因子：
4.1
通讯作者：
Li, Wensheng
中科院分区：
文献类型：
--
作者：
Tang, Zhiguo;Sun, Caiyun;Li, Wensheng
与哺乳动物一样，脂肪酸（FA）代谢在调节鱼类摄食中起着多种重要作用。多条证据表明，FA代谢对食物摄入的影响与虹鳟鱼下丘脑中神经肽Y（NPY）水平的变化有关。在哺乳动物中，有证据表明FA代谢通过下丘脑NPY调节摄食。因此，在脊椎动物中，NPY被认为是通过FA代谢介导食物摄入调节的重要因素。NPY对食物摄入的刺激作用是众所周知的。然而，据我们所知，NPY对下丘脑中FA代谢的影响尚未得到研究。本研究克隆了石斑鱼下丘脑中4种脂肪酸代谢关键酶的cDNA，并研究了能量状态和神经肽Y（NPY）对其mRNA表达的影响。从石斑鱼下丘脑中克隆了UCP2和CPT1a的全长cDNA以及ACC 1和FAS的部分编码序列。这些基因在下丘脑和胚胎发育的器官发生阶段表达。摄食节律研究表明，下丘脑NPY和CPT 1a的表达水平与摄食节律高度相关。长期禁食可显著诱导下丘脑NPY、CPT 1a和UCP 2的mRNA表达。体外研究表明，NPY以时间和剂量依赖性方式强烈刺激CPT 1a和UCP 2 mRNA表达。总的来说，这些结果表明，这四个基因相关的脂肪酸代谢可能发挥作用，在调节石斑鱼的食物摄入量，神经肽Y调节脂肪酸代谢石斑鱼下丘脑。这项研究首次在脊椎动物中显示了NPY对FA代谢相关酶基因表达的影响。(C)由Elsevier爱尔兰有限公司出版。
As in mammals, fatty acid (FA) metabolism plays diverse and vital roles in regulating food intake in fish. Multiple lines of evidence suggest that the effect of FA metabolism on food intake is linked to changes in the level of neuropeptide Y (NPY) in the hypothalamus of the rainbow trout. In mammals, the evidence suggests that FA metabolism regulates feeding via hypothalamic NPY. NPY is therefore considered an important factor that mediates the modulation of food intake by FA metabolism in vertebrates. The stimulatory effect of NPY on food intake is well known. However, to the best of our knowledge, the effect of NPY on FA metabolism in the hypothalamus has not been examined. In this study, we cloned the cDNA of four key enzymes involved in FA metabolism and assessed the effect of energy status and NPY on their mRNA expression in the hypothalamus of grouper. The full-length cDNAs of UCP2 and CPT1a and the partial coding sequence (CDS) of ACC1 and FAS were isolated from the grouper hypothalamus. These genes are expressed in the hypothalamus and during the organogenetic stage of embryogenesis. A feeding rhythm study showed that the hypothalamic expression level of NPY and CPT1a was highly correlated with feeding rhythm. Long-term fasting was found to significantly induce the hypothalamic mRNA expression of NPY, CPT1a and UCP2. An in vitro study demonstrated that NPY strongly stimulated CPT1a and UCP2 mRNA expression in a time- and dose-dependent manner. Collectively, these results suggest that these four genes related to FA metabolism may play a role in regulating food intake in grouper and, that NPY modulates FA metabolism in the grouper hypothalamus. This study showed, for the first time in vertebrates, the effect of NPY on the gene expression of FA metabolism-related enzymes. (C) 2013 Published by Elsevier Ireland Ltd.