Benzo(a)pyrene-albumin adducts in humans exposed to polycyclic aromatic hydrocarbons in an industrial area of Poland
Benzo(a)pyrene-albumin adducts in humans exposed to polycyclic aromatic hydrocarbons in an industrial area of Poland
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DOI：
10.1136/oem.54.9.662
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发表时间：
1997-09-01
影响因子：
4.9
通讯作者：
Haugen, A
中科院分区：
文献类型：
--
作者：
Kure, EH;Andreassen, A;Haugen, A
目的：测定苯并（a）芘与血清白蛋白的相互作用，试图确定实际暴露量，并评价白蛋白加合物测量作为暴露监测的生物标志物。方法：采用竞争性酶联免疫吸附测定法（ELISA）测定苯并（a）芘-二醇-环氧（BPDE）-白蛋白加合物来自三家工厂的焦炉工人和生活在波兰高度工业化地区的西里西亚的人们的血浆中。由于该地区空气中多环芳烃（PAHs）浓度较高，因此从波兰的一个农村非工业化地区选择了一个对照组。呼吸区空气测量的多环芳烃收集了一些participants.Results-Coke焦炉厂工人和非职业暴露的人有类似的浓度的白蛋白加合物，而农村控制显着较低（2.74 fmol加合物/亩白蛋白（SEM 0.124））。职业人群和环境人群血浆中BPDE-白蛋白加合物的平均浓度在夏季均显著升高（4.34 fmol加合物/μ g白蛋白（SEM 0.335）和4.55 fmol加合物/μ g白蛋白（SEM 0.296），分别）比在冬季样品（3.06 fmol加合物/μ g白蛋白（SEM 0.187）和3.04 fmol加合物/μ g白蛋白（SEM 0.184），分别），即使空气中的测量显示，在冬季的多环芳烃浓度较高。的统计分析没有显示任何影响的空气暴露对浓度的BPDE-白蛋白adducts. Conclusions，一个多元回归分析的测量浓度的BPDE-白蛋白加合物的所有组，在这两个季节，表明职业暴露没有贡献显着的加合物的形成。一般来说，白蛋白加合物的浓度在两个季节和不同参与者组之间的变化范围相对较小。没有发现极端的差异。
Objectives-The interaction of benzo(a)pyrene with serum albumin was measured in an attempt to identify the actual exposure and to evaluate albumin adduct measurements as biomarkers for exposure monitoring.Methods-Benzo(a)pyrene-diol-epoxide (BPDE)-albumin adducts were measured by competitive enzyme linked immunosorbent assay (ELISA) in plasma of coke oven plant workers from three plants and from people living in a highly industrialised area of Silesia in Poland. Due to the high air concentrations of polycyclic aromatic hydrocarbons (PAHs) in this area, a control group was selected from a rural non-industrialised area in Poland. Breathing zone air measurements of PAHs were collected from some of the participants.Results-Coke oven plant workers and non-occupationally exposed people had similar concentrations of albumin adducts whereas the rural controls were significantly lower (2.74 fmol adducts/mu g albumin (SEM 0.124)). The mean concentration of BPDE-albumin adduct in plasma of both the occupational and the environmental groups were significantly higher in the summer samples (4.34 fmol adducts/mu g albumin (SEM 0.335) and 4.55 fmol adducts/mu g albumin (SEM 0.296), respectively) than in the winter samples (3.06 fmol adducts/mu g albumin (SEM 0.187) and 3.04 fmol adducts/mu g albumin (SEM 0.184), respectively) even though the air measurements showed higher concentrations of PAHs in the winter. The statistical analysis did not show any effects of air exposures on concentrations of BPDE-albumin adduct.Conclusions-A multiple regression analysis of the measured concentrations of BPDE-albumin adducts for all the groups, during both seasons, indicates that occupational exposures do not contribute significantly to the formation of adducts. In general, the concentrations of albumin adducts found vary within relatively small limits for the two seasons and between the various groups of participants. No extreme differences were found.