Age-related changes in the intramuscular distribution of melanocytes in the Silky fowl
Age-related changes in the intramuscular distribution of melanocytes in the Silky fowl
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DOI：
10.1080/00071660600825082
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发表时间：
2006-08-01
影响因子：
2
通讯作者：
Iwamoto, H.
中科院分区：
文献类型：
--
作者：
Nishimura, S.;Oshima, I.;Iwamoto, H.
1．研究乌骨鸡骨骼肌黑素细胞分布特征与生长的关系。2．取1、3、5、10、20、30周龄乌骨鸡胸肌(PT)和髂胫束(ITL)肌肉称重，冰冻切片免疫组化检测I型胶原。 3．两块肌肉的I型胶原免疫阳性肌内膜和肌束膜均可见黑色素细胞。 4．图像分析显示，组织学切片中黑素细胞占据的总面积在1至3周内从PT肌肉的0.61%急剧下降至0.16%，ITL肌肉从1.67%下降至0.33%，然后逐渐下降。直到10周龄，ITL肌肉中的黑素细胞面积都大于PT肌肉中的黑色素细胞面积。5．我们得出的结论是，在发育早期阶段，乌骨鸡中肌内黑色素细胞的比例因不同类型的肌肉而异，并且随着生长而减少。
1. The characteristics of melanocyte distribution in skeletal muscles in the Silky fowl were investigated in association with growth.2. Pectoralis ( PT) and iliotibialis lateralis ( ITL) muscles from 1-, 3-, 5-, 10-, 20- and 30-week-old Silky males were weighed and collagen type I was detected in frozen sections immunohistochemically.3. Melanocytes were observed in the collagen type I-immunopositive endomysium and perimysium in both muscles.4. Image analysis indicated that the total area occupied by melanocytes in histological sections sharply decreased from 0.61% to 0.16% in PT muscle and from 1.67% to 0.33% in ITL muscle at 1 to 3 weeks, and then gradually decreased. The melanocyte area was larger in ITL muscle than in PT muscle until 10 weeks of age.5. We concluded that the proportion of intramuscular melanocytes in the Silky fowl differs between types of muscles in the early stages of development, and it decreases with growth.