The Expression Pattern of MicroRNAs and the Associated Pathways Involved in the Development of Porcine Placental Folds That Contribute to the Expansion of the Exchange Surface Area
The Expression Pattern of MicroRNAs and the Associated Pathways Involved in the Development of Porcine Placental Folds That Contribute to the Expansion of the Exchange Surface Area
复制标题
MicroRNA 的表达模式和参与猪胎盘褶皱发育的相关途径，有助于交换表面积的扩大
DOI：
10.1095/biolreprod.114.126540
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发表时间：
2015-09-01
影响因子：
3.6
通讯作者：
Yu, Mei
中科院分区：
文献类型：
--
作者：
Liu, Ruize;Wang, Min;Yu, Mei
猪弥漫性上皮性胎盘的显微镜折叠结构的发展是重要的，因为它扩大了母胎交换的表面积，从而提高了胎盘的效率。为了更好地了解这一过程的调控机制，我们对猪胎盘中胎盘褶皱发育起始和建立过程中的miRNA表达谱进行了表征。共发现了42个差异表达的mirna，并使用四种目标预测程序预测了它们的假定靶基因。通过与已发表的胎盘褶发育相应阶段的猪胎盘基因表达模式数据进行比较分析，仅保留与miRNA表达负相关的基因，用于进一步的功能和途径富集分析。结果表明，上调的mirna主要与细胞外基质重塑和组织形态发生相关，下调的mirna主要与细胞增殖和信号转导相关。此外，我们提供的证据表明，miR-130b可能通过抑制PPARG的表达来促进HPSE的表达，HPSE被报道为猪胎盘折叠的调节剂。此外，我们还揭示了在猪胎盘中表达的mirna靶对可能触发基质和基底膜的降解（miR-29a-COL1A2， COL3A1和LAMC1），并调节滋养层上皮细胞粘附连接（miR-200家族和miR-205-ZEB2-CDH1）和增殖（miR-17-92簇- hbp1和ULK1）。综上所述，这些结果表明mirna及其相关通路可能在猪胎盘褶发育中发挥潜在作用。
The development of the microscopically folded structure of the diffuse epitheliochorial placenta in pigs is important because it expands the surface area for maternal-fetal exchange, resulting in an increase in placental efficiency. To better understand the regulatory mechanisms involved in this process, we characterized miRNA expression profiles in porcine placentas during the initiation and establishment of placental fold development. A total of 42 miRNAs were found to be differentially expressed, and their putative target genes were predicted using four target prediction programs. Following a comparative analysis with published gene expression pattern data obtained from porcine placentas in the corresponding stages of placental fold development, only those genes that were negatively correlated with miRNA expression were retained for further function and pathway enrichment analysis. The results showed that the up-regulated miRNAs were associated mainly with extracellular matrix remodeling and tissue morphogenesis, while the down-regulated miRNAs were related to cell proliferation and signal transduction. Furthermore, we provide evidence that miR-130b may facilitate the expression of HPSE, which has been reported to be a regulator of the folding of the pig placenta, by suppressing the expression of PPARG. In addition, we also reveal that the miRNA-target pairs expressed in the pig placenta may trigger the degradation of the stromal matrix and basement membrane (miR-29a-COL1A2, COL3A1, and LAMC1) and regulate trophoblast epithelial cell adherens junctions (the miR-200 family and miR-205-ZEB2-CDH1) and proliferation (miR-17-92 cluster-HBP1 and ULK1). Taken together, these results indicate that miRNAs and related pathways may have potential roles in porcine placental fold development.