ISOLATION AND CHARACTERIZATION OF THE GENE FOR THE MURINE T-CELL DIFFERENTIATION ANTIGEN AND IMMUNOGLOBULIN-RELATED MOLECULE, LYT-2
ISOLATION AND CHARACTERIZATION OF THE GENE FOR THE MURINE T-CELL DIFFERENTIATION ANTIGEN AND IMMUNOGLOBULIN-RELATED MOLECULE, LYT-2
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DOI：
10.1093/nar/15.10.4337
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发表时间：
1987-05-26
影响因子：
14.9
通讯作者：
HERZENBERG, LA
中科院分区：
文献类型：
--
作者：
NAKAUCHI, H;TAGAWA, M;HERZENBERG, LA
我们在这里展示了5310碱基对Hind iii切割的基因组DNA片段的序列，其中包括Lyt-2基因，Lyt-2是一种小鼠分化抗原，在大多数未成熟T淋巴细胞以及细胞毒性抑制T细胞亚群上表达。我们还展示了Lyt-2的完整内含子/外显子结构。有五个外显子：一个融合的前导和免疫球蛋白可变区外显子，一个铰链区外显子，一个跨膜外显子和两个可选择剪接的胞质内外显子（这些外显子的可选择剪接产生38 kDa α Lyt-2和34 kDa α Lyt-2多肽）。启动子区域包含一个“TATA”框和与推定的免疫球蛋白转录控制元件cd/pd同源的序列。S1保护分析表明，胸腺细胞、淋巴结的T细胞和通过引入基因组DNA获得的Lyt-2转染物具有相同的起始位点。在3‘区，在长达700 bp的3’未翻译区之后存在一个聚腺苷化信号序列。
We present here the sequence of the 5310 base pair Hind III-cleaved genomic DNA segment that includes the gene for the Lyt-2, a murine differentiation antigen expressed on most immature T lymphocytes as well as the cytotoxic suppressor T cell subset. We also present the complete intron/exon structure of Lyt-2. There are five exons: a fused leader and immunoglobulin variable region like exon, a hinge region exon, a transmembrane exon and two alternatively spliced intracytoplasmic exons (alternative splicing of these exons yields the 38 kDa alpha Lyt-2 and 34 kDa alpha' Lyt-2 polypeptides). The promoter region contains a “TATA” box and sequences homologous to the putative immunoglobulin transcriptional control elements cd/pd. S1 protection analysis reveals that thymocytee, T cells from lymph nodes, and a Lyt-2 transfectant obtained by introduction of total genomic DNA have the same initiation site. In the 3' region, there is a polyadenylation signal sequence after a 700 bp long 3' untranslated region.