The Xanthomonas Type III Effector XopD Targets the Arabidopsis Transcription Factor MYB30 to Suppress Plant Defense (Retracted article. See vol. 30, pg. 253, 2018)
The Xanthomonas Type III Effector XopD Targets the Arabidopsis Transcription Factor MYB30 to Suppress Plant Defense (Retracted article. See vol. 30, pg. 253, 2018)
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DOI：
10.1105/tpc.111.088815
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发表时间：
2011-09-01
期刊：
影响因子：
11.6
通讯作者：
Rivas, Susana
中科院分区：
文献类型：
--
作者：
Canonne, Joanne;Marino, Daniel;Rivas, Susana
植物和动物病原体将 III 型效应子 (T3E) 注入宿主细胞以抑制宿主免疫并促进成功感染。 XopD 是一种来自 Xanthomonas Campestris pv vesicatoria 的 T3E，已被提议通过靶向植物转录因子和/或调节因子来促进细菌生长。在这里，我们发现来自 X.campestris pvcampestris B100 菌株的 XopD 能够靶向 MYB30，MYB30 是一种转录因子，通过转录激活与极长链脂肪酸 (VLCFA) 代谢相关的基因，正向调节拟南芥防御和对细菌的相关细胞死亡反应。 XopD 与 MYB30 特异性相互作用，从而抑制 MYB30 VLCFA 相关靶基因的转录激活并抑制拟南芥防御。 XopD 的螺旋-环-螺旋结构域对于介导这些作用是必要且充分的。这些结果说明了黄单胞菌开发的一种破坏植物防御并促进疾病发展的原始策略。
Plant and animal pathogens inject type III effectors (T3Es) into host cells to suppress host immunity and promote successful infection. XopD, a T3E from Xanthomonas campestris pv vesicatoria, has been proposed to promote bacterial growth by targeting plant transcription factors and/or regulators. Here, we show that XopD from the B100 strain of X. campestris pv campestris is able to target MYB30, a transcription factor that positively regulates Arabidopsis thaliana defense and associated cell death responses to bacteria through transcriptional activation of genes related to very-long-chain fatty acid (VLCFA) metabolism. XopD specifically interacts with MYB30, resulting in inhibition of the transcriptional activation of MYB30 VLCFA-related target genes and suppression of Arabidopsis defense. The helix-loop-helix domain of XopD is necessary and sufficient to mediate these effects. These results illustrate an original strategy developed by Xanthomonas to subvert plant defense and promote development of disease.