Side‐chain cleavage of phytosterols by Mycobacterium sp. MB 3683 in a biphasic ionic liquid/aqueous system
Side‐chain cleavage of phytosterols by Mycobacterium sp. MB 3683 in a biphasic ionic liquid/aqueous system
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DOI：
10.1002/jctb.4865
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发表时间：
2016-10
影响因子：
3.4
通讯作者：
Yuan Junjie;Y. Guan;Yan-Ting Wang;Hai-Qing Wang;Shanjing Yao
中科院分区：
文献类型：
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作者：
Yuan Junjie;Y. Guan;Yan-Ting Wang;Hai-Qing Wang;Shanjing Yao
背景植物甾醇的微生物转化已用于生产雄甾-4-烯-3,17-二酮（AD）。然而，底物溶解度低和产物抑制是有待解决的主要问题。双相系统是有效的策略，而离子液体（IL）作为双相系统中的相之一，由于环境限制，是传统有机溶剂的有前途的替代品。 结果在这项工作中，九种与水不混溶的离子液体被用于分枝杆菌的植物甾醇侧链裂解。 MB 3683。首先测量植物甾醇和 AD 在这些 IL 中的溶解度以及 AD 在水和 IL 之间的分配系数。 [PrMIM][PF6] 被确定为建立双相 IL-水系统的合适候选者。在最佳条件下，即接种量为 12%，48小时龄，IL 添加时间为 84小时，相比为 20:1（v/v，水/IL），在 200 的放大双相系统中，底物浓度为 5g L−1 的生物转化 5 天后，AD 产量达到 2.23 g L−1毫升。这种在双相IL-水系统中进行的生物转化比在单相水系统中进行的生物转化更有效。结论由于IL的环境优势和上述积极结果，双相IL-水系统有潜力应用于植物甾醇侧链裂解。 © 2015 中国化工学会
BACKGROUNDMicrobial transformation of phytosterols has been applied to produce androst‐4‐ene‐3,17‐dione (AD). However, low substrate solubility and product inhibition are major problems to be solved. Biphasic systems are efficient strategies, and ionic liquids (ILs), as one of the phases in biphasic systems, are promising alternatives to traditional organic solvents due to environmental constraints.RESULTSIn this work, nine water‐immiscible ILs were used in phytosterols side‐chain cleavage byMycobacteriumsp. MB 3683. The solubilities of phytosterols and AD in these ILs and distribution coefficients of AD between water and ILs were first measured. [PrMIM][PF6] was determined to be a suitable candidate to establish a biphasic IL–aqueous system. Under optimal conditions, i.e. inoculum size of 12% with 48 h age, IL addition at 84 h, and phase ratio of 20:1 (v/v, aqueous/IL), AD production reached 2.23 g L−1after 5 days of biotransformation with substrate concentration of 5 g L−1in a scaled‐up biphasic system of 200 mL. This bioconversion conducted in a biphasic IL–aqueous system was more efficient than that in a monophasic aqueous system.CONCLUSIONOwing to the environmental advantages of ILs and the above positive results, biphasic IL–aqueous systems have the potential to be applied in phytosterols side‐chain cleavage. © 2015 Society of Chemical Industry