USE OF MEMBRANE FILTER TECHNIQUE IN MICROBIOLOGICAL CONTROL FOR BREWING INDUSTRY
USE OF MEMBRANE FILTER TECHNIQUE IN MICROBIOLOGICAL CONTROL FOR BREWING INDUSTRY
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DOI：
10.1128/aem.15.4.736-737.1967
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发表时间：
1967-01-01
期刊：
影响因子：
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通讯作者：
NOBILE, J
中科院分区：
文献类型：
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作者：
NOBILE, J
建立了一种用膜过滤器连续过滤分离酵母细胞和细菌的物理方法。这样的方法消除了对抗生素的需要，以前需要允许这种群体的差异计数。通过使用合成培养基，成功地将过滤的所有酵母细胞保留并培养在1.2-[mu]膜滤器上。所有过滤的细菌避免了在1.2-[mu]膜滤器上的截留，并成功地在0.22-[mu]上培养。膜过滤器与相同的合成介质。当使用硫乙醇酸盐流体培养基检测时，这些系列过滤的最终滤液不含所有酵母细胞和细菌。
A physical method was developed involving serial filtration with membrane filters for separating yeast cells from bacteria. Such a method eliminates the need for antibiotics previously required to permit differential counting of such populations. All yeast cells filtered were successfully retained and cultivated on a 1.2-[mu] membrane filter by use of a synthetic medium. All bacteria filtered avoided entrapment on a 1.2-[mu] membrane filter and were successfully and cultivated on a 0.22-[mu]. membrane filter with the same synthetic medium. Final filtrates from these serial filtrations were free from all yeast cells and bacteria when tested with Fluid Thioglycollate Medium.