Purine nucleotides stimulate DNA synthesis in kidney epithelial cells in culture.
Purine nucleotides stimulate DNA synthesis in kidney epithelial cells in culture.
复制标题
嘌呤核苷酸刺激培养的肾上皮细胞中的 DNA 合成。
DOI：
10.1152/ajprenal.1985.249.6.f967
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Toback,FG
中科院分区：
文献类型：
--
作者：
Kartha,S;Toback,FG
腺嘌呤核苷酸注入急性肾功能衰竭的动物似乎可以促进肾功能和结构的恢复。为了确定这些化合物是否可以通过对肾细胞代谢的直接作用而起作用，在猴肾上皮细胞（BSC-1系）的培养物中评价了它们刺激DNA合成的能力。AMP和ADP使这些细胞的DNA合成比以前观察到的其他有丝分裂原增加了三倍。鸟苷和肌苷及其核苷酸和腺苷和ATP也有促有丝分裂作用，但程度较小，而嘧啶衍生物则无效。在AMP的存在下，[3 H]胸腺嘧啶标记的细胞的放射自显影表明，更多的细胞进入细胞周期的S期，细胞数量的评估显示增加的增殖。腺嘌呤核苷酸的促有丝分裂作用不能通过提高细胞内cAMP含量的药物重现，并且不依赖于血清。腺嘌呤核苷酸加入小鼠成纤维细胞培养物中时，不会改变DNA合成。这些结果表明，提供外源性嘌呤核苷和核苷酸刺激培养的肾上皮细胞中的DNA合成。
Adenine nucleotides infused into animals with acute renal failure appear to enhance recovery of kidney function and structure. To determine whether these compounds could act by a direct effect on renal cell metabolism, their capacity to stimulate DNA synthesis was evaluated in cultures of monkey kidney epithelial cells (BSC-1 line). AMP and ADP enhanced DNA synthesis by threefold more than was previously observed with other mitogens for these cells. Guanosine and inosine and their nucleotides and adenosine and ATP were also mitogenic but to a lesser extent, whereas pyrimidine derivatives were ineffective. In the presence of AMP, autoradiography of [3H]thymidine-labeled cells indicated that a greater number of cells entered the S phase of the cell cycle, and assessment of cell number revealed increased multiplication. The mitogenic effect of adenine nucleotides was not reproduced by agents that raise the cellular content of cAMP and was serum independent. Adenine nucleotides did not alter DNA synthesis when added to cultures of mouse fibroblasts. These results indicate that provision of exogenous purine nucleosides and nucleotides stimulate DNA synthesis in renal epithelial cells in culture.