Genome-wide identification and comparative expression analysis of NBS-LRR-encoding genes upon Colletotrichum gloeosporioides infection in two ecotypes of Fragaria vesca
Genome-wide identification and comparative expression analysis of NBS-LRR-encoding genes upon Colletotrichum gloeosporioides infection in two ecotypes of Fragaria vesca
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DOI：
10.1016/j.gene.2012.11.027
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发表时间：
2013-09-15
期刊：
影响因子：
3.5
通讯作者：
Gao, Qing-Hua
中科院分区：
文献类型：
--
作者：
Li, Jing;Zhang, Qing-Yu;Gao, Qing-Hua
炭疽病是世界范围内栽培草莓（Fragaria x ananassa Duchesne）的主要病害之一。NBS-LRR基因是已知最大的一类抗性基因，与草莓炭疽病抗性之间的相关性一直是难以捉摸的。在NCBI中进行BLAST检索，在草莓二倍体基因组中鉴定出94个FvNBS，其中67个为TIR-NBS-LRR型。至少36个FvNBS表达，其中25%为非编码基因。两个F结果表明，胡枝子HLJ和YW两个生态型在形态和生理反应上均表现出较大的差异。qRT-PCR结果显示，在12个叶片表达的FvNBS中，有5个表现出与C.两种生态型的胶孢菌侵染。这些结果表明，几种FvNBS的转录反应参与了胶孢镰刀菌的生态区特异性反应。维斯卡这些FvNBSs在鉴定草莓抗炭疽病分子组分和开发新的标记方面具有潜力。(C)2013 Elsevier B. V.保留所有权利。
Anthracnose caused by Colletotrichum spp. is one of the most destructive diseases of cultivated strawberry (Fragaria x ananassa Duchesne) worldwide. The correlation between NBS-LRR genes, the largest class of known resistance genes, and strawberry anthracnose resistance has been elusive. BLAST search in NCBI identified 94 FvNBSs in the diploid genome of strawberry Fragaria vesca, with 67 of the TIR-NBS-LRR type. At least 36 FvNBSs were expressed, with 25% being non-coding genes. Two F. vesca ecotypes, HLJ and YW, showed great variations in both morphological and physiological responses upon C gloeosporioides infection. qRT-PCR revealed that 5 of the 12 leaf-expressed FvNBSs displaying opposite transcription responses to C. gloeosporioides infection in two ecotypes. These results showed that the transcriptional responses of several FvNBSs were involved in the ecotype-specific responses to C gloeosporioides in F. vesca. These FvNBSs hold potential in characterizing molecular components and developing novel markers associated with anthracnose resistance in strawberry. (C) 2013 Elsevier B.V. All rights reserved.