ESTIMATION OF MEMBRANE-POTENTIALS OF INDIVIDUAL LYMPHOCYTES BY FLOW CYTOMETRY
ESTIMATION OF MEMBRANE-POTENTIALS OF INDIVIDUAL LYMPHOCYTES BY FLOW CYTOMETRY
复制标题
DOI：
10.1073/pnas.76.11.5728
复制
发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
KAMENTSKY, LA
中科院分区：
文献类型：
--
作者：
SHAPIRO, HM;NATALE, PJ;KAMENTSKY, LA
单个细胞的膜电位可以通过细胞摄取荧光亲脂性阳离子染料 3,3''-二己基氧杂碳花青碘化物的流式细胞术定量来估计。在泛影葡胺梯度上从外周血分离的人淋巴细胞被短杆菌肽均匀去极化并被缬氨霉素超极化。刀豆球蛋白 A 和植物血凝素仅使一小部分淋巴细胞去极化。流式细胞术技术可以精确检测对凝集素等刺激物的异质膜电位反应；它还可以为分选对给定刺激有不同反应的细胞提供基础。
The membrane potentials of individual cells could be estimated by flow cytometric quantitation of the cells'' uptake of the fluorescent lipophilic cationic dye 3,3''-dihexyloxacarbocyanine iodide. Human lymphocytes separated from peripheral blood on Hypaque-Ficoll gradients were uniformly depolarized by gramicidin and hyperpolarized by valinomycin. Concanavalin A and phytohemagglutinin depolarized only a fraction of the lymphocytes. The flow cytometric technique allowed precise detection of heterogeneous membrane potential responses to stimuli such as lectins; it could also provide a basis for sorting cells that respond differently to a given stimulus.