Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays.
Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays.
复制标题
活力试剂 PrestoBlue 与其他可用试剂在细胞毒性和抗菌性检测中的应用比较
DOI：
10.1155/2013/420601
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发表时间：
2013
影响因子：
3.4
通讯作者：
Berrington D
中科院分区：
文献类型：
--
作者：
Lall N;Henley-Smith CJ;De Canha MN;Oosthuizen CB;Berrington D
本研究比较了不同的市售活力试剂。生长指示试剂包括对碘硝基四氮唑紫（INT）、PrestoBlue和Alamar Blue，用于对变形链球菌、中间普氏菌、痤疮丙酸杆菌和结核杆菌进行抗菌分析。PrestoBlue和Alcohol Blue是基于刃天青的试剂，其导致快速且容易区分的颜色变化，从而允许视觉读数。INT和3′-[1-（苯基氨基-羰基）-3，4-四唑鎓]-双-[4-甲氧基-6-硝基]苯磺酸钠水合物（XTT）是基于四唑鎓的试剂，在存在代谢活性线粒体酶的情况下转化为甲瓒染料。对于细胞活力分析，比较试剂XTT和PrestoBlue。PrestoBlue能够清楚地指示各种阳性药物对照对各种微生物菌株的最小抑菌浓度（MIC）。PrestoBlue也是阳性药物对照对各种细胞系的50%抑制浓度（IC 50）的良好指标。
This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulted in a quick and easily distinguishable colour change that allowed for visual readings. INT and Sodium 3′-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate (XTT) are tetrazolium based reagents which are converted to a formazan dye in the presence of metabolically active mitochondria enzyme. For cell viability analysis, reagents XTT and PrestoBlue were compared. PrestoBlue was able to clearly indicate the minimum inhibitory concentration (MIC) of various positive drug controls on various microbial strains. PrestoBlue was also a good indicator of the 50% inhibitory concentration (IC50) of positive drug controls on various cell lines.