An important determinant of the ability of Turnip mosaic virus to infect Brassica spp. and/or Raphanus sativus is in its P3 protein
An important determinant of the ability of Turnip mosaic virus to infect Brassica spp. and/or Raphanus sativus is in its P3 protein
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DOI：
10.1099/vir.0.79825-0
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发表时间：
2004-07-01
影响因子：
3.8
通讯作者：
Okuda, S
中科院分区：
文献类型：
--
作者：
Suehiro, N;Natsuaki, T;Okuda, S
芜菁花叶病毒(TuMV，马铃薯Y病毒属，马铃薯Y病毒科)主要侵染十字花科植物。TuMV分离物Tu-3和Tu-2R1在甘蓝上表现出不同的侵染表型。和萝卜(Raphanus Sativus L.)从分离株Tu-3和Tu-2R1中分别构建了感染性全长cDNA克隆pTuC和pTuR1。侵染pTuC的后代病毒在感染的甘蓝上引起了系统性的黄化和环斑症状，但在萝卜上没有引起系统性的侵染。从感染pTuR1的植物中提取的病毒在甘蓝上诱导了轻微的褪绿斑点，并系统地感染萝卜诱导了花叶症状。通过在两个病毒分离物之间交换基因组片段，P3编码区被证明与TuMV系统感染以及它在甘蓝和萝卜上引起的症状有关。此外，P3区较小部分的交换导致了导致复杂感染表型的重组体，特别是pTuC衍生的N端序列和pTuR1衍生的C端序列的组合。组织免疫印迹分析表明，P3嵌合病毒的分布与亲本不同，P3组分的来源不仅影响病毒的积累，还影响病毒的远距离迁移。这些结果表明，P3蛋白在TuMV的感染循环中以及在决定该病毒和其他马铃薯Y病毒的寄主范围方面是一个重要的因素。
Turnip mosaic virus (TuMV, genus Potyvirus, family Potyviridae) infects mainly cruciferous plants. Isolates Tu-3 and Tu-2R1 of TuMV exhibit different infection phenotypes in cabbage (Brassica oleracea L.) and Japanese radish (Raphanus sativus L.). Infectious full-length cDNA clones, pTuC and pTuR1, were constructed from isolates Tu-3 and Tu-2R1, respectively. Progeny virus derived from infections with pTuC induced systemic chlorotic and ringspot symptoms in infected cabbage, but no systemic infection in radish. Virus derived from plants infected with pTuR1 induced a mild chlorotic mottle in cabbage and infected radish systemically to induce mosaic symptoms. By exchanging genome fragments between the two virus isolates, the P3-coding region was shown to be responsible for systemic infection by TuMV and the symptoms it induces in cabbage and radish. Moreover, exchanges of smaller parts of the P3 region resulted in recombinants that induced complex infection phenotypes, especially the combination of pTuC-derived N-terminal sequence and pTuR1-derived C-terminal sequence. Analysis by tissue immunoblotting of the inoculated leaves showed that the distributions of P3-chimeric viruses differed from those of the parents, and that the origin of the P3 components affected not only virus accumulation, but also long-distance movement. These results suggest that the P3 protein is an important factor in the infection cycle of TuMV and in determining the host range of this and perhaps other potyviruses.