MOLECULAR-CLONING OF PC3, A PUTATIVELY SECRETED PROTEIN WHOSE MESSENGER-RNA IS INDUCED BY NERVE GROWTH-FACTOR AND DEPOLARIZATION
MOLECULAR-CLONING OF PC3, A PUTATIVELY SECRETED PROTEIN WHOSE MESSENGER-RNA IS INDUCED BY NERVE GROWTH-FACTOR AND DEPOLARIZATION
复制标题
DOI：
10.1073/pnas.88.8.3353
复制
发表时间：
1991-04-01
影响因子：
11.1
通讯作者：
TIRONE, F
中科院分区：
文献类型：
--
作者：
BRADBURY, A;POSSENTI, R;TIRONE, F
PC3是神经生长因子在PC12细胞中诱导的即时早期基因，PC12细胞来源于肾上腺髓质肿瘤，在神经生长因子存在下经历神经元分化。这种诱导与新蛋白质合成无关，因为它可以在环己亚胺存在的情况下发生。通过膜去极化（体内和体外）和表皮生长因子，PC3也以类似的动力学诱导，但水平较低。成纤维细胞生长因子和白细胞介素6的诱导水平较低。在体内，它被发现在组织中表达，如胚胎13.5天的大脑、胎盘、羊膜和脾脏，它们正在增殖和/或分化。从cDNA中推断出的蛋白质序列表明存在信号肽，表明PC3是分泌的。
PC3 is an immediate early gene induced by nerve growth factor in PC12 cells, a cell line derived from a tumor of the adrenal medulla that undergoes neuronal differentiation in the presence of nerve growth factor. This induction is independent of new protein synthesis as it can occur in the presence of cycloheximide. PC3 is also induced with similar kinetics, but at lower levels, by membrane depolarization (both in vivo and in vitro) and epidermal growth factor. It is induced at much lower levels by fibroblast growth factor and interleukin 6. In vivo it is found expressed in tissues, such as brain at embryonic day 13.5, placenta, amnion, and spleen, which are proliferating and/or differentiating. The deduced protein sequence from the cDNA indicates the presence of a signal peptide, suggesting that PC3 is secreted.