CANINE-DISTEMPER TERMINAL AND INTERGENIC NONPROTEIN CODING NUCLEOTIDE-SEQUENCES - COMPLETION OF THE ENTIRE CDV GENOME SEQUENCE
CANINE-DISTEMPER TERMINAL AND INTERGENIC NONPROTEIN CODING NUCLEOTIDE-SEQUENCES - COMPLETION OF THE ENTIRE CDV GENOME SEQUENCE
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DOI：
10.1006/viro.1993.1103
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发表时间：
1993-03-01
期刊：
影响因子：
3.7
通讯作者：
UDEM, SA
中科院分区：
文献类型：
--
作者：
SIDHU, MS;HUSAR, W;UDEM, SA
分析了犬瘟热病毒(CDV)RNA聚合酶转录和复制功能的关键序列。该序列是使用基因组文库中的c-DNA克隆作为模板或者在大多数情况下使用基因组CDV RNA从聚合酶链式反应(PCR)产物中获得的。对编码CDV基因组3‘端和5’端的克隆进行了测序，并通过进一步的PCR实验对结果进行了验证。末端序列和两个非编码端的间隔的虚拟一致性说明了这些区域在复制和转录中的重要性。确定了每个CDV基因边界的序列，并将其与相关病毒进行了比较。这份报告完成了CDV基因组的序列测定。
Sequences critical for the transcription and replication functions of canine distemper virus (CDV) RNA polymerase were analyzed. The sequence was obtained from polymerase chain reaction (PCR) products using either c-DNA clones from a genomic library as template or in most instances genomic CDV RNA. Clones coding for the precise 3′- and 5′-ends of the CDV genome were sequenced and the results confirmed by additional PCR experiments. The virtual identity of terminal sequences and spacing at the two noncoding ends speak to the importance of these areas in replication and transcription. The sequence for each of the CDV gene boundaries was defined and all were compared to related viruses. This report completes the sequence determination of the CDV genome.