Early lesion formation in cattle experimentally infected with Mycobacterium bovis
Early lesion formation in cattle experimentally infected with Mycobacterium bovis
复制标题
牛实验性感染牛分枝杆菌后形成的早期病变
DOI：
10.1016/s0021-9975(98)80069-8
复制
发表时间：
1998-07-01
影响因子：
0.8
通讯作者：
Neill, SD
中科院分区：
文献类型：
--
作者：
Cassidy, JP;Bryson, DG;Neill, SD
在13头小牛中检查早期病变形成，这些小牛经鼻内接种2 × 10（7）个牛分枝杆菌菌落形成单位，并从接种后第3天（pid）至pid 42天（间隔小于或等于7天）单独或成对处死。在感染前后进行免疫学检查，并进行连续尸检。M.牛早在pid 3时从上呼吸道粘膜、咽后淋巴结和肺尾叶中回收。从pid 14开始处死的小牛的上呼吸道粘膜和肺部均检测到大体结核病变。在引流这些区域的淋巴结中也存在病变。在组织学检查中，中性粒细胞似乎在病变形成的最早阶段起关键作用，并且在pid 35时首次观察到病变矿化。病变和细胞免疫的同时发展，如体外淋巴细胞增殖和干扰素-γ测定反应所示，进一步证明了免疫致病机制在牛结核病发展中的作用。(C)1998年W.B.桑德斯有限公司
Early lesion formation was examined in 13 calves inoculated intranasally with 2 x 10(7) colony-forming units of Mycobacterium bovis and killed either singly or in pairs at intervals of less than or equal to 7 days from post-inoculation day (pid) 3 to pid 42. Immunological examinations were carried out before and after infection, and sequential necropsies were performed. M. bovis was recovered as early as pid 3, from the upper respiratory tract mucosae, retropharyngeal lymph nodes and caudal lung lobe. Gross tuberculous lesions were detected in both the upper respiratory tract mucosae and in the lungs of the calves killed from pid 14 onwards. Lesions were also present in the lymph nodes draining these areas. On histological examination, neutrophils appeared to play a key role in the earliest stages of lesion formation, and lesion mineralization was observed for the first time at pid 35. The contemporaneous development of lesions and cellular immunity, as demonstrated by in-vitro lymphocyte proliferation and interferon-gamma assay responses, provided further evidence of the role of immunopathogenic mechanisms in the development of bovine tuberculosis. (C) 1998 W.B. Saunders Company Limited.