Fatty acid binding protein expression in different human adipose tissue depots in relation to rates of lipolysis and insulin concentration in obese individuals
Fatty acid binding protein expression in different human adipose tissue depots in relation to rates of lipolysis and insulin concentration in obese individuals
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DOI：
10.1023/a:1020532823751
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发表时间：
2002-10-01
影响因子：
4.3
通讯作者：
Arner, P
中科院分区：
文献类型：
--
作者：
Fisher, RM;Thörne, A;Arner, P
两种脂肪酸结合蛋白（FABPs）在脂肪组织中表达，脂肪细胞脂质结合蛋白（ALBP）和角质形成细胞脂质结合蛋白（KLBP）。本研究调查了ABP在内脏和皮下人脂肪组织贮库中的表达，以及贮库和循环胰岛素浓度之间的脂解差异。ALBP和KLBP（蛋白质和RNA）在肥胖个体的皮下和网膜脂肪组织中定量，并相对于肌动蛋白表达。与网膜脂肪组织相比，皮下组织中ALBP RNA和蛋白表达显著更高（均p < 0.05），而KLBP RNA和蛋白表达在两个部位之间没有差异。血清胰岛素浓度与皮下和网膜脂肪组织中ALBP/KLBP RNA比值呈显著负相关（均p < 0.02）。在从皮下和网膜脂肪组织分离的脂肪细胞中测量的甘油和脂肪酸释放的基础速率在前者中显著更高（p 0.02）。因此，人脂肪组织的相对ALBP/KLBP含量在不同的脂肪组织库中是不同的，并且在RNA水平上与循环胰岛素浓度相关，至少在肥胖受试者中是这样。皮下脂肪细胞的基础脂解速率高于网膜脂肪组织，这可能与皮下脂肪细胞ALBP含量增加有关。因此，脂肪组织ABPs是一个有趣的候选调查，以进一步了解我们的胰岛素抵抗综合征和调节脂解。
Two fatty acid binding proteins (FABPs) are expressed in adipose tissue, adipocyte lipid binding protein ( ALBP) and keratinocyte lipid binding protein (KLBP). This study investigated ABP expression in visceral and subcutaneous human adipose tissue depots and associations with lipolytic differences between the depots and circulating insulin concentrations. ALBP and KLBP ( protein and RNA) were quantified in subcutaneous and omental adipose tissue from obese individuals and expressed relative to actin. ALBP RNA and protein expression was significantly higher in subcutaneous compared to omental adipose tissue ( both p < 0.05), whereas KLBP RNA and protein expression was no different between the two sites. There were significant inverse correlations between serum insulin concentrations and the ALBP/KLBP RNA ratio in both subcutaneous and omental adipose tissue ( both p < 0.02). Basal rates of glycerol and fatty acid release measured in adipocytes isolated from subcutaneous and omental adipose tissue were significantly higher in the former ( p 0.02). Therefore the relative ALBP/KLBP content of human adipose tissue is different in different adipose tissue depots and at the RNA level is related to the circulating insulin concentration, at least in obese subjects. The higher rates of basal lipolysis in adipocytes isolated from subcutaneous compared to omental adipose tissue might be related to the increased ALBP content of the former. Therefore adipose tissue ABPs are interesting candidates for investigation to further our understanding of the insulin resistance syndrome and regulation of lipolysis.