Comparative Proteomic Analysis Reveals Key Proteins Linked to the Accumulation of Soluble Sugars and Organic Acids in the Mature Fruits of the Wild Malus Species
Comparative Proteomic Analysis Reveals Key Proteins Linked to the Accumulation of Soluble Sugars and Organic Acids in the Mature Fruits of the Wild Malus Species
复制标题
比较蛋白质组学分析揭示了与野生苹果属成熟果实中可溶性糖和有机酸积累相关的关键蛋白质
DOI：
10.3390/plants8110488
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发表时间：
2019-11-01
期刊：
影响因子：
4.5
通讯作者：
Yuan, Yangyang
中科院分区：
文献类型：
--
作者：
Ma, Baiquan;Ding, Yuduan;Yuan, Yangyang
可溶性糖和有机酸是影响水果感官品质的主要因素。为了研究苹果果实可溶性糖和有机酸含量的相关基因，采用液相色谱-质谱/质谱联用技术对苹果属（Malus）2种苹果果实进行了无标记蛋白质组学分析。sargentii和M. niedzwetzkyana的可溶性糖和有机酸含量存在显著差异。共鉴定了13，036种独特的肽和1，079种差异表达的蛋白质。为了验证LC-MS/MS结果，通过平行反应监测进一步分析了五种候选蛋白质。结果与LC-MS/MS数据一致，证实了LC-MS/MS分析的可靠性。基于基因本体和京都基因与基因组百科全书（KEGG）数据库对差异表达蛋白进行功能注释，发现它们主要与生物学过程和细胞组分相关。此外，主要富集的KEGG途径与代谢过程有关。此外，31蛋白参与可溶性糖代谢，有机酸代谢，和H+-运输进行了鉴定。本研究结果为全面揭示苹果果实可溶性糖和有机酸含量的复杂调控机制提供了理论依据。
Soluble sugars and organic acids are the main determinants of fruit organoleptic quality. To investigate the genes responsible for the soluble sugar and organic acid contents of apple fruits, a label-free proteomic analysis involving liquid chromatography (LC)-mass spectrometry (MS)/MS was conducted with the fruits of two Malus species, M. sargentii and M. niedzwetzkyana, which exhibit significant differences in soluble sugar and organic acid contents. A total of 13,036 unique peptides and 1,079 differentially-expressed proteins were identified. To verify the LC-MS/MS results, five candidate proteins were further analyzed by parallel reaction monitoring. The results were consistent with the LC-MS/MS data, which confirmed the reliability of the LC-MS/MS analysis. The functional annotation of the differentially-expressed proteins, based on the gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, revealed that they were mainly related to biological processes and cellular components. Additionally, the main enriched KEGG pathways were related to metabolic processes. Moreover, 31 proteins involved in soluble sugar metabolism, organic acid metabolism, and H+-transport were identified. The results of this study may be useful for the comprehensive characterization of the complex mechanism regulating apple fruit-soluble sugar and organic acid contents.