Aberrant hippocampal mossy fiber sprouting correlates with greater NMDAR2 receptor staining
Aberrant hippocampal mossy fiber sprouting correlates with greater NMDAR2 receptor staining
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DOI：
10.1097/00001756-199604100-00015
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发表时间：
1996-04-10
期刊：
影响因子：
1.7
通讯作者：
Peacock, WJ
中科院分区：
文献类型：
--
作者：
Mathern, GW;Leite, JP;Peacock, WJ
这项研究在颞叶癫痫患者和注射海马内红藻氨酸 (KA) 的大鼠中确定了筋膜齿状分子层杂乱纤维出芽是否与 NMDAR2 免疫反应性 (IR) 的增加有关。将海马硬化患者 (n = 11) 与颞部肿块病变患者 (n = 7) 以及尸检获得的材料 (n = 4) 进行比较；将注射 KA 的单侧大鼠海马 (n = 7) 与注射生理盐水的对侧和未损伤的动物 (n = 7；对照) 进行比较。研究了海马的新蒂姆氏染色杂乱纤维出芽和 NMDAR2 IR。使用计算机图像分析将染色量化为灰度值（GV）。海马硬化患者和注射 KA 的大鼠表现出最大的内分子层 (IML) 杂乱纤维萌芽和 NMDAR2 染色。与尸检和有肿块病变的患者相比，海马硬化患者的 IML neo-Timm 染色（p = 0.0018）和 NMDAR2 染色（p = 0.0063）更高。同样，与对照组和注射盐水的大鼠相比，注射 KA 的海马显示出更大的 IML 杂乱纤维萌芽和 NMDAR2 IR (p = 0.0001)。此外，在人类和实验大鼠组中，IML 杂乱纤维发芽与更大的 IML NMDAR2 染色呈正相关 (p < 0.0099)。这些结果支持这样的假设：在严重受损的海马中，异常杂乱的纤维出芽和 IML NMDAR2 受体染色的一致增加可能有助于或部分解释颗粒细胞过度兴奋和海马癫痫的病理生理学。
THIS study determined in temporal lobe epilepsy patients and rats injected with intrahippocampal kainate (KA) whether fascia dentata molecular layer messy fiber sprouting was associated with increases in NMDAR2 immunoreactivity (IR). Patients with hippocampal sclerosis (n = 11) were compared with those with temporal mass lesions (n = 7) and material obtained at autopsies (n = 4); and unilateral KA-injected rat hippocampi (n = 7) were compared with the contralateral saline-injected side and non-lesioned animals (n = 7; control). Hippocampi were studied for neo-Timm's stained messy fiber sprouting and NMDAR2 IR. The staining was quantified as gray values (GV) using computer image analysis. Hippocampal sclerosis patients and KA-injected rats showed the greatest inner molecular layer (IML) messy fiber sprouting and NMDAR2 staining. Compared with autopsies and patients with mass lesions, hippocampal sclerosis patients had greater IML neo-Timm's (p = 0.0018) and NMDAR2 staining (p = 0.0063). Similarly, compared with controls and saline-injected rats, KA-injected hippocampi showed greater IML messy fiber sprouting and NMDAR2 IR (p = 0.0001). Furthermore, IML messy fiber sprouting positively correlated with greater IML NMDAR2 staining in both human and experimental rat groups (p < 0.0099). These results support the hypothesis that in severely damaged hippocampi abnormal messy fiber sprouting and concordant increases in IML NMDAR2 receptor staining may contribute or partially explain granule cell hyperexcitability and the pathophysiology of hippocampal epilepsy.