Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26
Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26
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DOI：
10.1073/pnas.94.14.7446
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发表时间：
1997-07-08
影响因子：
11.1
通讯作者：
Smith, GR
中科院分区：
文献类型：
--
作者：
Fox, ME;Virgin, JB;Smith, GR
粟酒裂殖酵母M26减数分裂重组热点的活性取决于七聚体5 '-ATGACGT-3'的存在。含有ade 6-M26基因的DNA片段到其他染色体位点的易位先前已经证明，七聚体在一些但不是所有的易位中起作用，这表明热点活性取决于染色体背景。在本研究中，通过使用定点诱变在基因组中的新位置产生七聚体序列，在不存在总DNA变化的情况下测试热点活性。当在ade 6和ural基因中通过1-4 bp的诱变产生时，七聚体在所有测试的位置以方向无关的方式作为重组热点是有活性的。因此，七聚体序列可以在其他染色体环境中产生活性热点，前提是总的染色体结构没有改变;这一结果与M26热点活性所需的特定高阶染色质结构的假设一致。
The activity of the M26 meiotic recombination hot spot of Schizosaccharomyces pombe depends on the presence of the heptamer 5'-ATGACGT-3'. Transplacement of DNA fragments containing the ade6-M26 gene to other chromosomal loci has previously demonstrated that the heptamer functions in some, but not all, transplacements, suggesting that hot spot activity depends on chromosomal context. In this study, hot spot activity was tested in the absence of gross DNA changes by using site-directed mutagenesis to create the heptamer sequence at novel locations in the genome. When created by mutagenesis of 1-4 bp in the ade6 and ural genes, the heptamer was active as a recombination hot spot, in an orientation-independent manner, at all locations tested. Thus, the heptamer sequence can create an active hot spot in other chromosomal contexts, provided that the gross chromosomal structure is not altered; this result is consistent with the hypothesis that a specific higher-order chromatin structure is required for M26 hot spot activity.