Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes
Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes
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DOI：
10.1007/s003359901112
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发表时间：
1999-09-01
期刊：
影响因子：
2.5
通讯作者：
Bird, A
中科院分区：
文献类型：
--
作者：
Hendrich, B;Abbott, C;Bird, A
DNA甲基化是小鼠发育所必需的，并且与基因表达的控制有关。MeCP2、MBD1。MBD 2、MBD 3和MBD 4包含哺乳动物核蛋白家族，其通过在每个氨基酸基序中存在称为甲基-CpG结合结构域（MBD）而相关。除MBD 3外，这些蛋白质中的每一种都能够特异性结合甲基化DNA。MeCP 2、MBD 1和MBD 2也可以抑制转录。我们描述了人类和小鼠Mbd 1，Mbd 2，Mbd 3和Mbd 4基因的基因组结构和染色体定位。我们发现，高度相似的MBD 2和MBD 3蛋白质由映射到人类和小鼠不同染色体的基因编码，但显示出相似的基因组结构。相比之下，Mbd 1和Mbd 2基因一起映射到鼠和人的染色体（Chrs）18。Mbd 3和Mbd 4基因分别定位于鼠Chrs 10和6，而人MBD 3和MBD 4基因分别定位于Chrs 19和3。
DNA methylation is essential for murine development and is implicated in the control of gene expression. MeCP2, MBD1. MBD2, MBD3, and MBD4 comprise a family of mammalian, nuclear proteins related by the presence in each of an amino acid motif called the methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MeCP2, MBD1 and MBD2 can also repress transcription. We describe the genomic structure and chromosomal localization of the human and murine Mbd1, Mbd2, Mbd3, and Mbd4 genes. We find that the highly similar MBD2 and MBD3 proteins are encoded by genes that map to different chromosomes in humans and mice but show a similar genomic structure. The Mbd1 and Mbd2 genes, in contrast, map together to murine and human Chromosomes (Chrs)18. The Mbd3 and Mbd4 genes map to murine Chrs 10 and 6, respectively, while the human MBD3 and MBD4 genes map to Chrs 19 and 3, respectively.