Monocyte chemoattractant protein-1 levels in patients with glomerular disease
Monocyte chemoattractant protein-1 levels in patients with glomerular disease
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DOI：
10.1016/s0272-6386(96)90097-9
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发表时间：
1996-05-01
影响因子：
13.2
通讯作者：
Tan, LC
中科院分区：
文献类型：
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作者：
Rovin, BH;Doe, N;Tan, LC
趋化因子如单核细胞趋化蛋白-1（MCP-1）和白细胞介素-8已被牵涉到在免疫性肾损伤期间将白细胞募集到肾小球。为了检测MCP-1在人类肾小球疾病中的表达，我们测量了各种肾小球疾病患者的尿MCP-1水平。提示肾小球疾病患者尿中存在MCP-1，炎症性肾小球疾病患者尿MCP-1水平较高，尿MCP-1水平与蛋白尿程度相关（r = 0.71，P < 0.0001），而与血清MCP-1水平无相关性（r = 0.14，P > 0.3）。尿中MCP-1具有生物学活性，在微量趋化试验中可增加单核细胞的迁移，这种活性可通过向样品中加入抗MCP-1抗体而减弱。计数肾小球巨噬细胞证实肾小球炎症与尿MCP-1水平相关（r = 0.53，P < 0.02），光镜下肾小球损伤严重者，肾小球呈束状、新月形、坏死，毛细血管内增生）的患者尿液中MCP-1水平显著高于肾小球病变不严重的患者（1，962 +/-612 pg MCP-1/mg肌酐对314 +/-45 pg MCP-1/mg肌酐; P < 0.002）。总之，这些结果表明，尿MCP-1反映了肾小球中产生的MCP-1参与肾小球炎症反应的程度。(C)1996年由国家肾脏基金会，公司。
Chemokines such as monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 have been implicated in recruiting leukocytes to the glomerulus during immune renal injury, To detect the expression of MCP-1 in human glomerular disease, we measured urinary MCP-1 levels in patients with a variety of glomerulopathies. These data demonstrated that MCP-1 was present in the urine of patients with glomerular disease and that patients with inflammatory glomerulopathies had higher levels of urinary MCP-1, Urinary MCP-1 correlated with the extent of proteinuria (r = 0.71, P < 0.0001), but not with serum MCP-1 levels (r = 0.14, P > 0.3). The MCP-1 present in urine was biologically active, increasing monocyte migration in a microchemotaxis assay, This activity was attenuated by the addition of anti-MCP-l antibody to the samples, Enumerating glomerular macrophages demonstrated that glomerular inflammation was correlated with urinary MCP-1 levels (r = 0.53, P < 0.02), Individuals with light microscopic evidence of severe glomerular injury tie, crescents, necrosis, endocapillary proliferation) had significantly higher levels of MCP-1 in their urine than patients with less severe glomerular changes (1,962 +/- 612 pg MCP-1/mg creatinine v 314 +/- 45 pg MCP-1/mg creatinine; P < 0.002), Taken together, these results suggest that urinary MCP-1 reflects the extent to which MCP-1 that is produced in the glomerulus participates in the glomerular inflammatory response. (C) 1996 by the National Kidney Foundation, Inc.