Development of an Electrochemical Immunosensor for Salivary Oxytocin Determination
Development of an Electrochemical Immunosensor for Salivary Oxytocin Determination
复制标题
用于测定唾液催产素的电化学免疫传感器的开发
DOI：
10.1109/bmeicon.2018.8609952
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kudo Hiroyuki
中科院分区：
文献类型：
--
作者：
Asano Airi;Shiibashi Mako;Kudo Hiroyuki
采用竞争性酶联免疫吸附法（ELISA）构建了一种小型化的电化学-催产素（OXT）传感器并进行了测试。OXT传感器测量OXT作为葡萄糖氧化酶标记的催产素的减少，其竞争性地被抗OXT抗体捕获。我们采用硝化纤维素膜作为抗体的载体，因为硝化纤维素膜比用微量滴度板作为载体的输出信号提高了两倍。在实验中，首先使用常规生物素化捕获抗体和亲和素- god光学测量竞争性免疫反应。结果，证实了通过构建免疫系统来测量催产素是可能的。之后，使用碳石墨电极在电极上对催产素进行电化学测量。氧化还原电流与催产素浓度相关，校准范围为15pg/mL，据报道为唾液催产素。
A miniaturized electrochemical-oxytocin (OXT) sensor using competitive ELISA was constructed and tested. The OXT sensor measures OXT as reduction of glucose oxidase-labeled oxytocin, which competitively captured by anti-OXT antibodies. We employed a nitrocellulose membrane as a support for antibody because the nitrocellulose membrane improved the output signal by two folds comparing with using a microtiter plate as a support. In the experiment, competitive immunoreaction was first optically measured using conventional biotinylated capture antibody and avidin-GOD. As a result, it was confirmed that oxytocin measurement was possible by the constructed immune system. After that, oxytocin was electrochemically measured on the electrode using a carbon graphite electrode. Redox currents showed a relationship with oxytocin concentration and the calibration range involved 15pg/mL, which has been reported as salivary oxytocin.