Microcystin LR degradation by Pseudomonas aeruginosa alkaline protease
Microcystin LR degradation by Pseudomonas aeruginosa alkaline protease
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DOI：
10.1016/s0045-6535(97)00002-7
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发表时间：
1997-02-01
期刊：
影响因子：
8.8
通讯作者：
Watanabe, MF
中科院分区：
文献类型：
--
作者：
Takenaka, S;Watanabe, MF
我们从日本一个湖泊的表层水中分离出几种细菌。铜绿假单胞菌是唯一能在体外降解微囊藻毒素LR的细菌。微囊藻毒素LR在铜绿假单胞菌培养液中的降解率为4.5%，并被代谢为（2S，3S，8 S）-3-氨基-2，6，8-三甲基-10-十一碳-4E，6E-二烯酸（DmADDA）。铜绿假单胞菌可能通过亲核方式水解微囊藻毒素LR的肽键。我们研究了绿脓杆菌产生的绿脓菌螯铁蛋白、绿脓菌蓝蛋白和碱性蛋白酶是否影响微囊藻毒素LR的还原。结果表明，在100 μ M H_2O_2和100 μ M绿脓菌螯铁蛋白、绿脓菌素或两者的存在下，由微囊藻毒素LR产生DmADDA。然而，DmADDA的产生很少（2至12摩尔%）。在用铜绿假单胞菌碱性蛋白酶处理后，从微囊藻毒素LR产生75摩尔%的DmADDA。因此，铜绿假单胞菌碱性蛋白酶是降解微囊藻毒素的主要酶。(C)1997 Elsevier Science Ltd.
We isolated several species of bacteria from the surface water of a Japanese lake. Of the isolated bacteria, Pseudomonas aeruginosa was the only species which could degrade microcystin LR in vitro. Microcystin LR decreased to 4.5% of the spiked microcystin LR quantity in the P. aeruginosa culture, and was metabolized to (2S, 3S, 8S)-3-amino-2, 6, 8-trimethyl-10-ehenyldeca-4E, 6E-dienoic acid (DmADDA). It was possible that P. aeruginosa hydrolysed nucleophilically the peptide bond of microcystin LR. We examined if pyochelin, pyocyanin and alkaline protease produced by P. aeruginosa affected the reduction of microcystin LR. In the result, DmADDA was produced from microcystin LR in the presence of 100 mu M H2O2 and 100 mu Meach of pyochelin, pyocyanin or both. However, the production of DmADDA was slight(2 to 12 mole%). After treatment with P. aeruginosa alkaline protease, DmADDA was produced 75 mole% from microcystin LR. Therefore, we concluded that microcystin was degraded mainly by the action of P. aeruginosa alkaline protease. (C) 1997 Elsevier Science Ltd.