Identification of hypervariable and conserved regions in the surface envelope gene in the bovine lentivirus.
Identification of hypervariable and conserved regions in the surface envelope gene in the bovine lentivirus.
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DOI：
10.1006/viro.1995.1532
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发表时间：
1995-10
期刊：
影响因子：
3.7
通讯作者：
D. Suarez;C. Whetstone
中科院分区：
文献类型：
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作者：
D. Suarez;C. Whetstone
比较了牛慢病毒（BIV）9个不同分离株的表面囊膜（SU）基因核苷酸和推导的氨基酸（aa）序列的多样性。对来源于原始参考菌株R29的分离株和BIV田间分离株进行了分析。鉴定了6个保守区和6个高变区。许多高变区位于SU蛋白表面的预测区域。所有菌株之间的SU基因比较显示高达50%的氨基酸序列的分歧。当逆转录酶基因的保守区域之间进行比较的8个分离株，有不到11%的氨基酸序列的分歧。当比较所有分离株时，在第2高变区（V2）中观察到SU基因的最大大小差异，最大和最小变异体之间的差异高达104-aa。R29-106是BIV原始分离株的感染性分子克隆，与原型分离株R29-127相比，在V2中具有87-bp的缺失。本研究测序的所有R29衍生分离株的SU基因大小与R29-106相似。本研究测序的4个田间分离株的SU基因大于R29-127。R29衍生分离株可能不代表目前存在于美国牛中的BIV。
The surface envelope (SU) gene of nine different isolates of the bovine lentivirus (BIV) were compared for nucleotide and deduced amino acid (aa) sequence diversity. Analyses were done both on isolates derived from the original reference strain, R29, and on field isolates of BIV. Six conserved and six hypervariable regions were identified. Many of the hypervariable regions were located in areas predicted to be on the surface of the SU protein. The SU gene comparison among all isolates showed up to a 50% aa sequence divergence. When a conserved region of the reverse transcriptase gene was compared among eight of the isolates, there was less than 11% aa sequence divergence. When comparing all isolates, the greatest size differences in the SU gene are observed in the 2nd hypervariable region (V2) with up to a 104-aa difference between the largest and smallest variant. R29-106, an infectious molecular clone of the original isolate of BIV, has an 87-bp deletion in V2 as compared to prototype isolate R29-127. All R29-derived isolates sequenced for this study had a SU gene size similar to R29-106. The four field isolates sequenced for this study had SU genes larger than R29-127. R29-derived isolates may not be representative of BIV currently present in United States cattle.