Functional analysis of Foxp3 and its mutants by retroviral transduction of murine primary CD4(+) T Cells.
Functional analysis of Foxp3 and its mutants by retroviral transduction of murine primary CD4(+) T Cells.
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通过逆转录病毒转导小鼠原代 CD4( ) T 细胞对 Foxp3 及其突变体进行功能分析。
DOI：
10.1007/978-1-0716-2647-4_7
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发表时间：
2023
影响因子：
--
通讯作者：
Hori S
中科院分区：
文献类型：
--
作者：
Nakajima A;Murakami R;Hori S
转录因子Foxp3/ Foxp3通过与许多靶基因和伴侣蛋白相互作用来协调T （Treg）细胞的发育和功能。对自然发生或工程Foxp3/ Foxp3突变的功能分析为了解复杂的Foxp3/ Foxp3分子网络如何运作提供了重要的见解。在这里，我们描述了小鼠原代常规CD4+T细胞逆转录病毒转导的详细方案，以确定Foxp3突变对treg细胞样表型和Foxp3赋予的功能的影响。
The transcription factor Foxp3/FOXP3 orchestrates regulatory T (Treg) cell development and function by interacting with numerous target genes and partner proteins. Functional analysis of naturally occurring or engineered Foxp3/FOXP3 mutations has provided important insights into how the complex Foxp3/FOXP3-centered molecular network operates. Here, we describe detailed protocols for retroviral transduction of murine primary conventional CD4+T cells to determine the impacts of Foxp3 mutations on the Treg-cell-like phenotype and function conferred by Foxp3.