An idiotypic replica of carcinoembryonic antigen inducing cellular and humoral responses directed against human colorectal tumours
An idiotypic replica of carcinoembryonic antigen inducing cellular and humoral responses directed against human colorectal tumours
复制标题
癌胚抗原的独特型复制品诱导针对人类结直肠肿瘤的细胞和体液反应
DOI：
10.1002/ijc.2910500525
复制
发表时间：
1992
影响因子：
6.4
通讯作者：
R. Robins
中科院分区：
文献类型：
--
作者：
L. Durrant;G. Denton;E. Jacobs;M. Mee;R. Moss;E. Austin;R. W. Baldwin;J. Hardcastle;R. Robins
制备了单克隆抗独特型抗体（抗Id MAb）708（IgG2b），其可抑制NCRC23（IgG1）MAb与CEA结合，并阻止放射性标记的CEA与MAb NCRC23结合。未观察到该抗独特型抗体识别其他3种抗CEA抗体、其他3种IgG1或2种IgM MAb。当使用免疫印迹技术时，708抗Id MAb未能与分离的MAb NCRC 23重链或轻链结合，而与完整抗体结合。用708抗Id MAb免疫小鼠、大鼠和人淋巴细胞（体外），所得Ab3抗体均抑制标记的708抗Id MAb与MAb NCRC 23的结合，并与CEA反应，表明708抗Id MAb诱导抗CEA抗体应答。类似地，用708抗Id MAb免疫的小鼠可以在体外用CEA或表达CEA的肿瘤细胞再刺激，其诱导特异性T细胞增殖应答。从结直肠肿瘤中分离的人肿瘤浸润淋巴细胞或癌症患者的外周血T细胞在体外用708抗Id MAb或不相关的IgG2b抗体刺激。6天后，用CEA再刺激两组淋巴细胞，并且用708抗Id MAb致敏的淋巴细胞响应于CEA而增殖。这些结果表明，708抗Id单克隆抗体可以作为CEA的独特型复制品，刺激细胞和体液抗CEA免疫应答。因此，它是一个巨大的利益作为一个独特型疫苗对结直肠癌。
A monoclonal anti‐idiotypic antibody (anti‐Id MAb) 708 (IgG2b), which inhibited the binding of NCRC23 (IgG1) MAb to CEA and prevented radiolabelled CEA from binding to MAb NCRC23, was produced. No recognition of 3 other anti‐CEA antibodies, 3 other IgG1 or 2 IgM MAbs was observed with this anti‐idiotypic antibody. When an immunoblotting technique was used, 708 anti‐Id MAb failed to bind to isolated heavy or light chains of MAb NCRC23, whereas binding was observed with intact antibody. Mouse, rat and human lymphocytes (in vitro) were immunized with 708 anti‐Id MAb and the resultant Ab3 antibodies all inhibited binding of labelled 708 anti‐Id MAb to MAb NCRC23 and also reacted with CEA, showing that 708 anti‐Id MAb induced anti‐CEA antibody responses. Similarly, mice immunized with 708 anti‐Id MAb could be restimulated in vitro with either CEA or tumour cells expressing CEA which induced specific T‐cell proliferative responses. Human tumour‐infiltrating lymphocytes isolated from colorectal tumours or peripheral blood T cells from cancer patients were stimulated in vitro with 708 anti‐Id MAb or an irrelevant IgG2b antibody. Six days later both sets of lymphocytes were restimulated with CEA, and lymphocytes primed to 708 anti‐Id MAb proliferated in response to CEA. These results suggest that 708 anti‐Id MAb can act as an idiotypic replica of CEA and stimulate cellular and humoral anti‐CEA immune responses. It is therefore of great interest as an idiotypic vaccine against colorectal cancer.
登录
查看更多内容
癌症患者的抗独特型免疫：免疫反应的调节。
DOI：
10.1073/pnas.84.22.8055
发表时间：
1987
影响因子：
11.1
作者：
Herlyn,D;Wettendorff,M;Schmoll,E;Iliopoulos,D;Schedel,I;Dreikhausen,U;Raab,R;Ross,AH;Jaksche,H;Scriba,M
通讯作者：
Scriba,M
鼠单克隆抗独特型抗体作为人类癌胚抗原的潜在网络抗原。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bhattacharya-Chatterjee,M;Mukerjee,S;Biddle,W;Foon,KA;Kohler,H
通讯作者：
Kohler,H
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Raychaudhuri,S;Kang,CY;Kaveri,SV;Kieber-Emmons,T;Kohler,H
通讯作者：
Kohler,H
通过非内部图像 (Ab2 α) 抗独特型抗体在小鼠中诱导抗乙型肝炎表面抗原反应。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schick,MR;Dreesman,GR;Kennedy,RC
通讯作者：
Kennedy,RC
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Raychaudhuri,S;Saeki,Y;Chen,JJ;Iribe,H;Fuji,H;Kohler,H
通讯作者：
Kohler,H