The Arabidopsis Somatic Embryogenesis Receptor Kinase 1 Gene Is Expressed in Developing Ovules and Embryos and Enhances Embryogenic Competence in Culture
The Arabidopsis Somatic Embryogenesis Receptor Kinase 1 Gene Is Expressed in Developing Ovules and Embryos and Enhances Embryogenic Competence in Culture
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DOI：
10.1104/pp.127.3.803
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发表时间：
2001-11
期刊：
影响因子：
7.4
通讯作者：
V. Hecht;Jean-Philippe Vielle-Calzada;M. Hartog;E. Schmidt;K. Boutilier;U. Grossniklaus;S. Vries
中科院分区：
文献类型：
--
作者：
V. Hecht;Jean-Philippe Vielle-Calzada;M. Hartog;E. Schmidt;K. Boutilier;U. Grossniklaus;S. Vries
我们在这里报告的分离拟南芥体细胞胚胎发生受体样激酶1（AtSERK 1）基因，我们证明了它的作用，在建立体细胞胚胎发生在文化。AtSERK 1基因在培养物中的胚胎发生细胞形成期间和早期胚胎发生期间高度表达。AtSERK 1基因首先在植物中大孢子发生期间在发育中的胚珠的核中、在功能性大孢子中以及在胚囊的所有细胞中表达直至受精。受精后，AtSERK 1表达见于发育胚胎的所有细胞中，直到心脏阶段。在这个阶段之后，AtSERK 1表达在胚胎或发育中的种子的任何部分中不再可检测到。在成体血管组织中检测到低表达。花椰菜花叶病毒35 S启动子控制下的全长AtSERK 1 cDNA的异位表达没有导致任何改变的植物表型。然而，过表达AtSERK 1 mRNA的幼苗表现出3- 4倍的体细胞胚胎发生启动效率的增加。因此，增加的AtSERK 1水平足以在培养中赋予胚胎发生能力。
We report here the isolation of the Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1 (AtSERK1) gene and we demonstrate its role during establishment of somatic embryogenesis in culture. The AtSERK1 gene is highly expressed during embryogenic cell formation in culture and during early embryogenesis. The AtSERK1 gene is first expressed in planta during megasporogenesis in the nucleus of developing ovules, in the functional megaspore, and in all cells of the embryo sac up to fertilization. After fertilization, AtSERK1 expression is seen in all cells of the developing embryo until the heart stage. After this stage, AtSERK1 expression is no longer detectable in the embryo or in any part of the developing seed. Low expression is detected in adult vascular tissue. Ectopic expression of the full-length AtSERK1 cDNA under the control of the cauliflower mosaic virus 35S promoter did not result in any altered plant phenotype. However, seedlings that overexpressed the AtSERK1 mRNA exhibited a 3- to 4-fold increase in efficiency for initiation of somatic embryogenesis. Thus, an increased AtSERK1 level is sufficient to confer embryogenic competence in culture.