Effect of RNA interference of BID and BAX mRNAs on apoptosis in granulosa cell-derived KGN cells.
Effect of RNA interference of BID and BAX mRNAs on apoptosis in granulosa cell-derived KGN cells.
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DOI：
10.1262/jrd.11-121h
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发表时间：
2012-02
期刊：
影响因子：
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通讯作者：
T. Sai;Fuko Matsuda;Y. Goto;A. Maeda;M. Sugimoto;Hong-mei Gao;A. M. Kabir;Junyou Li;N. Manabe
中科院分区：
文献类型：
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作者：
T. Sai;Fuko Matsuda;Y. Goto;A. Maeda;M. Sugimoto;Hong-mei Gao;A. M. Kabir;Junyou Li;N. Manabe
在线粒体依赖性II型细胞凋亡中，bh3相互作用结构域死亡激动剂（BID）和bcl -2相关X蛋白（BAX）促进死亡配体和受体介导的细胞死亡。在猪卵巢中，卵泡颗粒细胞的BID和BAX水平在闭锁期间升高。为了证实BID和BAX在颗粒细胞中的促凋亡活性，我们利用人卵巢颗粒瘤细胞系KGN检测了BID或BAX的RNA干扰对细胞凋亡的影响。通过逆转录聚合酶链反应（RT-PCR）和Western blotting检测KGN细胞中BID和BAX的表达。然后，我们使用小干扰RNA （siRNA）抑制KGN细胞中BID和BAX mRNA的表达。当BID或BAX被抑制时，凋亡细胞率明显降低。在颗粒源性细胞中，BID和BAX表现出促凋亡活性。这些结果表明，BID和BAX在线粒体依赖性II型细胞凋亡中起信号转导因子的作用。
In mitochondrion-dependent type II apoptosis, BH3-interacting domain death agonist (BID) and BCL-2-associated X protein (BAX) promote death ligand and receptor-mediated cell death. In porcine ovaries, the levels of BID and BAX increase in follicular granulosa cells during atresia. In the present study, to confirm the pro-apoptotic activity of BID and BAX in granulosa cells, we examined the effect of RNA interference of BID or BAX on apoptosis using a human ovarian granulosa tumor cell line, KGN. By reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, expression of BID and BAX was detected in KGN cells. Then, we suppressed BID and BAX mRNA expression in KGN cells using small interfering RNA (siRNA). When BID or BAX was suppressed, a significant decrease in the apoptotic cell rate was noted. In granulosa-derived cells, BID and BAX showed pro-apoptotic activity. These results suggest that BID and BAX act as signal-transducing factors in mitochondrion-dependent type II apoptosis.