Influence of dietary taurine and housing density on oviduct function in laying hens
Influence of dietary taurine and housing density on oviduct function in laying hens
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日粮牛磺酸和饲养密度对蛋鸡输卵管功能的影响
DOI：
10.1631/jzus.b1400256
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发表时间：
2015-06-01
影响因子：
5.1
通讯作者：
Miao, Jin-feng
中科院分区：
文献类型：
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作者：
Dai, Bin;Zhang, Yuan-shu;Miao, Jin-feng
本试验旨在研究日粮牛磺酸水平和饲养密度对蛋鸡输卵管功能的影响。将绿壳蛋鸡随机分为1个散养组和2个笼养组，1个为低密度组，1个为高密度组。每组又分为对照组（C）和牛磺酸治疗组（T）。除T组日粮中添加0.1%牛磺酸外，其余各组基础日粮相同。试验持续15 d。记录存活率、产蛋率、每日饲料消耗量和每日增重。组织学变化，炎症介质水平，氧化和抗氧化水平进行了测定。结果表明，日粮中补充牛磺酸和降低饲养密度显着衰减输卵管的病理生理变化。与其他两个饲养组相比，高密度饲养组的核因子-κ B（NF-κ B）DNA结合活性显着增加，并通过补充牛磺酸降低。肿瘤坏死因子-α（TNF-α）mRNA表达在高密度和低密度C、T组均显著增加。在自由范围和低密度组，饮食牛磺酸显着降低TNF-α mRNA的表达。补充牛磺酸降低干扰素-γ（IFN-γ）mRNA的表达显着的低密度组。白细胞介素4（IL-4）mRNA表达在笼养母鸡中显著升高。高密度C组IL-10 mRNA表达高于散养组和低密度C组。添加牛磺酸显著降低高密度组鸡IL-10 mRNA表达，提高自由放养鸡超氧化物歧化酶（SOD）活性。我们得出结论，牛磺酸对输卵管损伤具有重要的保护作用。降低住房密度还导致更少的氧化应激，更少的炎性细胞浸润和更低水平的炎症介质在输卵管中。因此，日粮中添加牛磺酸和降低鸡舍密度均能改善蛋鸡输卵管损伤，促进输卵管健康，提高产蛋量。
Experiments were conducted to study the effects of dietary taurine and housing density on oviduct function in laying hens. Green-shell laying hens were randomly assigned to a free range group and two caged groups, one with low-density and the other with high-density housing. Each group was further divided into control (C) and taurine treatment (T) groups. All hens were fed the same basic diet except that the T groups' diet was supplemented with 0.1% taurine. The experiment lasted 15 d. Survival rates, laying rates, daily feed consumption, and daily weight gain were recorded. Histological changes, inflammatory mediator levels, and oxidation and anti-oxidation levels were determined. The results show that dietary taurine supplementation and reduced housing density significantly attenuated pathophysiological changes in the oviduct. Nuclear factor-kappa B (NF-kappa B) DNA binding activity increased significantly in the high-density housing group compared with the two other housing groups and was reduced by taurine supplementation. Tumor necrosis factor-alpha (TNF-alpha) mRNA expression in the high-density and low-density C and T groups increased significantly. In the free range and low-density groups, dietary taurine significantly reduced the expression of TNF-alpha mRNA. Supplementation with taurine decreased interferon-gamma (IFN-gamma) mRNA expression significantly in the low-density groups. Interleukin 4 (IL-4) mRNA expression was significantly higher in caged hens. IL-10 mRNA expression was higher in the high-density C group than in the free range and low-density C groups. Supplementation with taurine decreased IL-10 mRNA expression significantly in the high-density group and increased superoxide dismutase (SOD) activity in the free range hens. We conclude that taurine has important protective effects against oviduct damage. Reducing housing density also results in less oxidative stress, less inflammatory cell infiltration, and lower levels of inflammatory mediators in the oviduct. Therefore, both dietary taurine and reduced housing density can ameliorate oviduct injury, enhance oviduct health, and promote egg production in laying hens.