DISTRIBUTION OF SCHISTOSOME GENETIC DIVERSITY WITHIN MOLLUSCAN INTERMEDIATE HOSTS
DISTRIBUTION OF SCHISTOSOME GENETIC DIVERSITY WITHIN MOLLUSCAN INTERMEDIATE HOSTS
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DOI：
10.1017/s0031182000064970
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发表时间：
1995-08-01
期刊：
影响因子：
2.4
通讯作者：
DESOUZA, CP
中科院分区：
文献类型：
--
作者：
MINCHELLA, DJ;SOLLENBERGER, KM;DESOUZA, CP
在巴西贝洛奥里藏特附近的两个地点收集了自然感染的光滑双脐螺，并用从单个螺中分离出的曼氏血吸虫尾蚴来感染个体小鼠。单个蠕虫 DNA 的遗传比较是通过 Southern 印迹与多态性重复 DNA 元件的杂交来完成的。寄生虫个体的基因谱揭示了自然感染的中间宿主中多种寄生虫基因型。观察到的中间宿主中血吸虫基因型的分布表明，超过一半的受感染蜗牛具有多种毛蚴。蜗牛携带多种感染的可能性比偶然预期的更大。这种程度的过度分散与高水平的遗传变异性相结合，促进了多基因型的传播，并有助于维持寄生虫的遗传多样性。
Naturally infected Biomphalaria glabrata snails were collected at two sites near Belo Horizonte, Brazil, and Schistosoma mansoni cercariae isolated from single snails were used to infect individual mice. Genetic comparison of single worm DNA was accomplished by hybridization of Southern blots to a polymorphic repetitive DNA element. Genetic profiles of parasite individuals revealed a diverse array of parasite genotypes in naturally infected intermediate hosts. The observed distribution of schistosome genotypes among intermediate hosts indicates that over half of the infected snails harbour multiple miracidia. Snails were more likely to carry multiple infections than expected by chance. This degree of overdispersion combined with high levels of genetic variability facilitates multi-genotype transmission and helps maintain parasite genetic diversity.