Selective increment of phosphatidylserine on the autophagic body membrane in yeast’s vacuole.
Selective increment of phosphatidylserine on the autophagic body membrane in yeast’s vacuole.
复制标题
酵母真空中自噬体膜上磷脂酰丝氨酸的选择性增量。
DOI：
10.1002/1873-3468.14167
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Yamakuchi;Y.;Kurokawa;Y.;Konishi;R.;Fukuda;K.;Masatani;T.;Fujita;A.
在酵母细胞中，自噬体是一种双膜结构;内膜成为液泡中的自噬体膜。自噬酶降解自噬体。没有关于其选择性降解的关键信息。利用电子显微镜方法，四个磷脂分布在自噬体和自噬体膜上的自噬诱导。磷脂酰丝氨酸（PtdSer）的标记在自噬体膜显着增加后，它从自噬体转换，但保持低的空泡膜。自噬体膜上PtdSer在atg 15酵母中也有增加。这些结果表明，PtdSer的选择性增加的自噬体，而不是空泡，膜，可以解释的自噬膜的选择性降解。
In yeast cells, the autophagosome is a double‐membrane structure; the inner membrane becomes the autophagic body membrane in the vacuole. Vacuolar enzymes degrade the autophagic body. There is no critical information regarding its selective degradation. Using the electron microscopy method, distributions of four phospholipids were examined in the autophagosomal and autophagic body membranes upon autophagy induction. The labeling of phosphatidylserine (PtdSer) in the autophagic body membrane dramatically increased after it converted from the autophagosome, but remained low in the vacuolar membrane. PtdSer in the autophagic body membrane also increased inatg15∆ yeast. These results suggest that the selective increment of PtdSer in the autophagic body, but not the vacuolar, membrane, can explain the selective degradation of the autophagic membrane.